Chaudhary Dhiraj Kumar, Kim Jaisoo
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2211-2218. doi: 10.1099/ijsem.0.001925. Epub 2017 Jul 12.
During a study of psychrophilic hydrocarbon-degrading bacteria, two yellow, Gram-staining-negative, non-motile, and rod shaped bacteria, designated R-10-9T and K-4-2 were isolated from oil-contaminated soil from Ulaanbaatar, Mongolia. Both strains were able to grow at 0-32 °C, pH 5.0-9.5, and 0-1 % (w/v) NaCl concentration. These strains were taxonomically characterized by a polyphasic approach. On the basis of the results of 16S rRNA gene sequence analysis, R-10-9T and K-4-2 belong to the genus Flavobacterium and are closely related to Flavobacterium oncorhynchi 631-08T (97.80 and 98.09 % sequence similarity, respectively). The pairwise sequence similarity between R-10-9T and K-4-2 was observed to be 99.72 %. In both strains, the predominant respiratory quinone was menaquinone-6; the major polar lipid was phosphatidylethanolamine; and the major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 0, C16 : 0 3-OH, antesio-C15 : 0, C16 : 0, iso-C17 : 0 3-OH, and iso-C15 : 0 3-OH. The genomic DNA G+C contents of R-10-9T and K-4-2 were 35.9 and 35.4 mol%, respectively. The DNA-DNA relatedness between R-10-9T and K-4-2 was higher than 70 % but relatedness values with closely related reference strains were less than 35 %. The morphological, physiological, chemotaxonomic, and phylogenetic analyses clearly distinguished R-10-9T from its closest phylogenetic neighbours. Thus, R-10-9T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium olei sp. nov. is proposed. The type strain is R-10-9T (=KEMB 9005-447T=KACC 18997T=JCM 31673T), and strain K-4-2 as an additional strain.
在一项对嗜冷烃降解细菌的研究中，从蒙古乌兰巴托受石油污染的土壤中分离出两株黄色、革兰氏染色阴性、无运动性的杆状细菌，分别命名为R-10-9T和K-4-2。两株菌都能在0-32 °C、pH 5.0-9.5和0-1 %（w/v）NaCl浓度下生长。采用多相分类法对这些菌株进行了分类学特征分析。基于16S rRNA基因序列分析结果，R-10-9T和K-4-2属于黄杆菌属，与虹鳟黄杆菌631-08T密切相关（序列相似性分别为97.80%和98.09 %）。观察到R-10-9T和K-4-2之间的成对序列相似性为99.72 %。在两株菌中，主要呼吸醌为甲基萘醌-6；主要极性脂为磷脂酰乙醇胺；主要脂肪酸为总和特征3（C16 : 1ω7c和/或C16 : 1ω6c）、异C15 : 0、C16 : 0 3-OH、anteiso-C15 : 0、C16 : 0、异C17 : 0 3-OH和异C15 : 0 3-OH。R-10-9T和K-4-2的基因组DNA G+C含量分别为35.9和35.4 mol%。R-10-9T和K-4-2之间的DNA-DNA相关性高于70 %，但与密切相关的参考菌株的相关性值低于35 %。形态学、生理学、化学分类学和系统发育分析清楚地将R-10-9T与其最密切的系统发育邻居区分开来。因此，R-10-9T代表黄杆菌属的一个新物种，为此提出名称油黄杆菌（Flavobacterium olei sp. nov.）。模式菌株为R-10-9T（=KEMB 9005-447T=KACC 18997T=JCM 31673T），菌株K-4-2作为另一菌株。
