Dahal Ram Hari, Chaudhary Dhiraj Kumar, Kim Jaisoo
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Aug;67(8):2653-2659. doi: 10.1099/ijsem.0.002000. Epub 2017 Aug 15.
Two strains of bacteria designated CB-3T and CB-31 were isolated from Kyonggi University forest soil. Cells were aerobic, Gram-stain-negative, oxidase-positive, non-motile, non-spore-forming, rod-shaped and yellow-pigmented. They were able to grow at 15-42 °C, pH 5.5-9.5 and with 0-1.5 % (w/v) NaCl concentration. Flexirubin-type pigments were absent. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains CB-3T and CB-31 formed a lineage within the family Flavobacteriaceae of the phylum Bacteroidetes that was distinct from various species of the genus Flavobacterium, including Flavobacterium humicola UCM-46T (99.58 % sequence similarity), Flavobacterium suncheonense GH29-5T (98.12 %), Flavobacterium pedocola UCM-R36T (97.57 %), Flavobacterium fulvum UCM-R15T (96.8 %) and Flavobacterium cauense R2A-7T (96.31 %). Both strains contained MK-6 as the sole quinone. The major polar lipid was phosphatidylethanolamine. The major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 0 3-OH, summed feature 9 (iso-C17 : 1ω9c and/or C16 : 0 10-methyl) and iso-C15 : 1 G. The DNA G+C content of the strains was 35.8-36.7 mol%. DNA-DNA relatedness between strain CB-3T and the most closely related members of the genus Flavobacterium ranged from 32 % to 59 %. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished strains CB-3T and CB-31 from their closest phylogenetic neighbours. Thus, strains CB-3T and CB-31 represent a novel species of the genus Flavobacterium, for which the name Flavobacterium flaviflagrans sp. nov. is proposed. The type strain is CB-3T (=KEMB 9005-535T=KACC 19112T=NBRC 112704T).
从庆熙大学森林土壤中分离出两株细菌，分别命名为CB-3T和CB-31。细胞为需氧型，革兰氏染色阴性，氧化酶阳性，不运动，不产芽孢，杆状且呈黄色色素沉着。它们能够在15-42 °C、pH 5.5-9.5以及NaCl浓度为0-1.5 %（w/v）的条件下生长。不存在柔韧菌素型色素。基于16S rRNA基因序列的系统发育分析表明，菌株CB-3T和CB-31在拟杆菌门黄杆菌科内形成了一个分支，该分支与黄杆菌属的各种物种不同，包括土栖黄杆菌UCM-46T（序列相似性为99.58 %）、顺天黄杆菌GH29-5T（98.12 %）、土壤黄杆菌UCM-R36T（97.57 %）、暗黄杆菌UCM-R15T（96.8 %）和考恩斯黄杆菌R2A-7T（96.31 %）。两株菌株均以MK-6作为唯一醌类。主要极性脂为磷脂酰乙醇胺。主要细胞脂肪酸为异-C15 : 0、异-C17 : 0 3-OH、异-C15 : 0 3-OH、总和特征9（异-C17 : 1ω9c和/或C16 : 0 10-甲基）和异-C15 : 1 G。这些菌株的DNA G+C含量为35.8-36.7 mol%。菌株CB-3T与黄杆菌属最密切相关成员之间的DNA-DNA相关性在32 %至59 %之间。形态学、生理学、化学分类学和系统发育分析清楚地将菌株CB-3T和CB-31与其最密切的系统发育邻居区分开来。因此，菌株CB-3T和CB-31代表黄杆菌属的一个新物种，为此提出新名称黄褐黄杆菌（Flavobacterium flaviflagrans sp. nov.）。模式菌株为CB-3T（=KEMB 9005-535T=KACC 19112T=NBRC 112704T）。
