Kingsley-Hickman P, Sako E Y, Andreone P A, St Cyr J A, Michurski S, Foker J E, From A H, Petein M, Ugurbil K
FEBS Lett. 1986 Mar 17;198(1):159-63. doi: 10.1016/0014-5793(86)81204-2.
Using 31P NMR and the saturation-transfer method, the unidirectional rate of ATP synthesis was measured in isolated, Langendorff-perfused, isovolumic rat hearts operating at a rate pressure product of 25.6 +/- 2.5 (SE) X 10(3) mmHg X min-1 and consuming O2 at a rate of 35 +/- 2 mumol O2 X min-1 X (g dry wt)-1, at 37 degrees C. This rate was 7.2 +/- 0.9 mumol X s-1 X (g dry wt)-1 and was related to the rate of oxygen atom consumption by a ratio of 6.3 +/- 0.9. These data show that in the intact heart the unidirectional rate of ATP synthesis exceeds the net rate of ATP synthesis and consumption by approximately a factor of 2.
采用31P核磁共振和饱和转移方法，在37℃下，对处于心率血压乘积为25.6±2.5（标准误）×10³mmHg×min⁻¹、耗氧速率为35±2μmol O₂×min⁻¹×（干重g）⁻¹的离体Langendorff灌注等容大鼠心脏的ATP合成单向速率进行了测量。该速率为7.2±0.9μmol×s⁻¹×（干重g）⁻¹，与氧原子消耗速率的比值为6.3±0.9。这些数据表明，在完整心脏中，ATP合成单向速率超过ATP合成与消耗的净速率约2倍。
