Shenyang Pharmaceutical University , Shenyang 110016, China.
Bijie Municipal Hospital of Traditional Chinese Medicine , Bijie 551700, China.
J Agric Food Chem. 2017 Aug 2;65(30):6211-6221. doi: 10.1021/acs.jafc.7b02550. Epub 2017 Jul 21.
The polyphenolic profiles of four berries (blueberry, bilberry, mulberry, and cranberry) in China were investigated using Fourier transform-ion cyclotron resonance mass spectrometry (FT-ICR MS). Thirty-nine polyphenols including 26 anthocyanins, 9 flavonoids, and 4 phenolic acids were identified accurately. Cyanidin aglycones are common in four berries, and malvidin aglycones are the main compounds found in bilberry and cranberry. The anthocyanin level in blueberry are the highest with 739.6 ± 17.14 mg/g DW and presented the strongest antioxidant capacity in DPPH, ABTS, FRAP, and ORAC assay. In α-glycosidase, the inhibition activity was in the following order: mulberry > bilberry > blueberry > cranberry. For the PTP1B inhibition assay, blueberry demonstrated the highest inhibitory effect with IC 3.06 ± 0.02 μg/mL, followed by bilberry, mulberry, and cranberry. Molecular docking results showed that cyanidin aglycones had the highest inhibition activity to PTP1B.
采用傅里叶变换离子回旋共振质谱（FT-ICR MS）研究了中国 4 种浆果（蓝莓、越橘、桑椹和蔓越莓）的多酚谱。准确鉴定了 39 种多酚，包括 26 种花色苷、9 种类黄酮和 4 种酚酸。四种浆果中均含有常见的矢车菊苷元，而飞燕草苷元是越橘和蔓越莓中的主要化合物。蓝莓中的花色苷含量最高，为 739.6 ± 17.14mg/g DW，在 DPPH、ABTS、FRAP 和 ORAC 测定中表现出最强的抗氧化能力。在α-葡萄糖苷酶抑制活性方面，其抑制活性顺序为：桑椹＞越橘＞蓝莓＞蔓越莓。在 PTP1B 抑制测定中，蓝莓表现出最高的抑制效果，IC 3.06 ± 0.02μg/mL，其次是越橘、桑椹和蔓越莓。分子对接结果表明，矢车菊苷元对 PTP1B 具有最高的抑制活性。
