Fang Bao-Zhu, Han Ming-Xian, Liu Lan, Zhang Zi-Tong, Liu Wei-Liang, Shen Jing-Ting, Wang Yan, Zhang Wan-Qin, Wei Da-Qiao, Li Wen-Jun
State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, PR China.
Medical Faculty of Kunming University of Science and Technology, Kunming University of Science and Technology, Kunming, PR China.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2357-2362. doi: 10.1099/ijsem.0.001958. Epub 2017 Jul 12.
A novel actinobacterial strain, designated SYSU K10001T, was isolated from a limestone sample collected from a karst cave in Xingyi county, Guizhou province, south-western China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic and Gram-stain-positive. On the basis of 16S rRNA gene sequence analysis, strain SYSU K10001T was most closely related to the type strains of the genus Lentzea, Lentzea albida NBRC 16102T (98.8 % similarity) and Lentzea waywayandensis NRRL B-16159T (98.6 %), and is therefore considered to represent a member of the genus Lentzea. DNA-DNA hybridization values between strain SYSU K10001T and related type strains of the genus Lentzea were less than 70 %. In addition, meso-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The whole-cell sugars were arabinose, fructose, mannose and xylose. The major isoprenoid quinone was MK-9(H4), while the major fatty acids (>10 %) were iso-C16 : 0 and C14 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositol mannoside, one unidentified phospholipid and one unidentified lipid. The genomic DNA G+C content of strain SYSU K10001T was 69.4 mol%. On the basis of phenotypic, genotypic and phylogenetic data, strain SYSU K10001T represents a novel species of the genus Lentzea, for which the name Lentzea cavernae sp. nov. is proposed. The type strain is SYSU K10001T (=KCTC 39804T=CGMCC 4.7367T=NBRC 112394T).
从中国西南部贵州省兴义县一个喀斯特洞穴采集的石灰岩样本中分离出一株新的放线菌菌株，命名为SYSU K10001T。采用多相分类法对该菌株的分类地位进行了研究。该菌株细胞为需氧型，革兰氏染色阳性。基于16S rRNA基因序列分析，菌株SYSU K10001T与Lentzea属的模式菌株Lentzea albida NBRC 16102T（相似度98.8%）和Lentzea waywayandensis NRRL B - 16159T（相似度98.6%）关系最为密切，因此被认为是Lentzea属的一个成员。菌株SYSU K10001T与Lentzea属相关模式菌株之间的DNA - DNA杂交值小于70%。此外，中 - 二氨基庚二酸是细胞壁肽聚糖中的诊断性二氨基酸。全细胞糖为阿拉伯糖、果糖、甘露糖和木糖。主要类异戊二烯醌为MK - 9(H4)，而主要脂肪酸（>10%）为异C16 : 0和C14 : 0。极性脂为双磷脂酰甘油、磷脂酰乙醇胺、羟基磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、一种未鉴定的磷脂和一种未鉴定的脂类。菌株SYSU K10001T的基因组DNA G + C含量为69.4 mol%。基于表型、基因型和系统发育数据，菌株SYSU K10001T代表Lentzea属的一个新物种，为此提出名为Lentzea cavernae sp. nov. 的新名称。模式菌株为SYSU K10001T（=KCTC 39804T=CGMCC 4.7367T=NBRC 112394T）。
