Im Wan-Taek, Yi Kwon-Jung, Lee Sang-Suk, Moon Hyung In, Jeon Che Ok, Kim Dong-Woon, Kim Soo-Ki
Department of Biotechnology, Hankyoung National University, 327 Chungang-no Anseong-si, Kyonggi-do 17579, Republic of Korea.
Department of Animal Science and Technology, Konkuk University, Seoul 05029, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2343-2348. doi: 10.1099/ijsem.0.001955. Epub 2017 Jul 12.
A Gram-stain-positive, oxidase- and catalase-positive, aerobic, rod-shaped bacterium, designated strain SK-3146T, was isolated from animal feed. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, revealed that the strain formed a distinct lineage within the genus Paenibacillus that was closely related to Paenibacillusyunnanensis JCM 30953T (98.6 %), Paenibacillusvulneris CCUG 53270T (98.0 %) and Paenibacilluschinjuensis DSM 15045T (96.9 %). Cells were non-motile, endospore-forming and formed milky colonies on NA and R2A agar media. Growth of strain SK-3146T occurred at temperatures of 18-45 °C, at pH 6.0-9.5 and between 0.5-3.0 % NaCl (w/v). The major menaquinone was MK-7, with lesser amounts of MK-6 present. The cell wall peptidoglycan of strain SK-3146T contained meso-diaminopimelic acid. The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 53.8 mol% and the DNA-DNA hybridization relatedness values between strain SK-3146T and P.yunnanensis JCM 30953T and P.vulneris CCUG 53270T were 26.13±0.8 % and 38.7±0.6 %, respectively. The phenotypic, phylogenetic and chemotaxonomic results indicate that strain SK-3146T represents a novel species of the genus Paenibacillus, for which the name Paenibacillus konkukensis sp. nov. is proposed. The type strain is SK-3146T (=KACC 18876T=LMG 29568T).
从动物饲料中分离出一株革兰氏染色阳性、氧化酶和过氧化氢酶阳性、需氧的杆状细菌，命名为SK-3146T菌株。基于16S rRNA基因序列比较的系统发育分析表明，该菌株在芽孢杆菌属内形成了一个独特的分支，与云南芽孢杆菌JCM 30953T（98.6%）、脆弱芽孢杆菌CCUG 53270T（98.0%）和晋州芽孢杆菌DSM 15045T（96.9%）密切相关。细胞无运动性，形成芽孢，在NA和R2A琼脂培养基上形成乳白色菌落。SK-3146T菌株在18-45℃、pH 6.0-9.5和0.5-3.0%（w/v）NaCl条件下生长。主要甲基萘醌为MK-7，同时存在少量MK-6。SK-3146T菌株的细胞壁肽聚糖含有内消旋二氨基庚二酸。主要脂肪酸为anteiso-C15:0和iso-C16:0。主要极性脂为双磷脂酰甘油和磷脂酰乙醇胺。DNA G+C含量为53.8 mol%，SK-3146T菌株与云南芽孢杆菌JCM 30953T和脆弱芽孢杆菌CCUG 53270T之间的DNA-DNA杂交相关性值分别为26.13±0.8%和38.7±0.6%。表型、系统发育和化学分类学结果表明，SK-3146T菌株代表芽孢杆菌属的一个新物种，为此提出新名称韩国芽孢杆菌（Paenibacillus konkukensis sp. nov.）。模式菌株为SK-3146T（=KACC 18876T=LMG 29568T）。
