Kaur Navjot, Seuylemezian Arman, Patil Prashant P, Patil Prabhu, Krishnamurti Srinivasan, Varelas Joseph, Smith David J, Mayilraj Shanmugam, Vaishampayan Parag
1Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Chandigarh 160 036, India.
2Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA 91109, USA.
Int J Syst Evol Microbiol. 2018 Oct;68(10):3190-3196. doi: 10.1099/ijsem.0.002967. Epub 2018 Aug 21.
A Gram-stain-positive, motile, endospore-producing, facultative anaerobic bacterial strain, designated ATCC 27380, was isolated from heat-stressed soil of Cape Canaveral, Florida, USA. Growth was observed at 20-42 °C (optimum, 37 °C), at pH 6.0-10.0 (optimum pH 7.0) and in the presence of 0.5-3 % NaCl (optimum 0.5 %). The cell wall contained meso-diaminopimelic acid as the diagnostic amino acid and the isoprenoid quinone was MK-7. The polar lipids present were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and one unknown phospholipid. The main fatty acids were iso-C15 : 0 and anteiso-C15 : 0. Phylogenetic analysis based on 16S rRNA gene sequencing affiliated strain ATCC 27380 to the genus Paenibacillus, and showed the highest sequence similarity to Paenibacillus rigui JCM 16352 (97.0 %). The other closely related type strains exhibited 16S rRNA gene sequence similarity values below 95.9 %. The draft genome of ATCC 27380 had a size of 4,361,187 bases, with a G+C content of 51.0 %. The average nucleotide identity and in silico DNA-DNA hybridization values between strain ATCC 27380 and P. rigui JCM 16352 were 72.5% and 18.5 %, respectively, which were below the threshold suggested for species differentiation (96% and 70 %, respectively). The average amino acid identity between strain ATCC 27380 and P. rigui JCM 16352 was 68.72 %, which was above the suggested genus level demarcation of 65 %. Based on phenotypic, genotypic and chemotaxonomic data, strain ATCC 27380 represents a novel species in the genus Paenibacillus, for which the name Paenibacillusxerothermodurans sp. nov. (=DSM 520=NRRL NRS-1629=ATCC 27380) is proposed.
从美国佛罗里达州卡纳维拉尔角受热胁迫的土壤中分离出一株革兰氏阳性、具运动性、产芽孢的兼性厌氧细菌菌株，命名为ATCC 27380。该菌株在20-42 °C（最适温度为37 °C）、pH 6.0-10.0（最适pH 7.0）以及0.5-3 % NaCl（最适浓度0.5 %）条件下可生长。细胞壁含有内消旋二氨基庚二酸作为诊断性氨基酸，类异戊二烯醌为MK-7。存在的极性脂质有磷脂酰甘油、磷脂酰乙醇胺、双磷脂酰甘油和一种未知磷脂。主要脂肪酸为异-C15 : 0和anteiso-C15 : 0。基于16S rRNA基因测序的系统发育分析将菌株ATCC 27380归为芽孢杆菌属，与里氏芽孢杆菌JCM 16352的序列相似性最高（97.0 %）。其他密切相关的模式菌株16S rRNA基因序列相似性值低于95.9 %。ATCC 27380的基因组草图大小为4,361,187个碱基，G+C含量为51.0 %。菌株ATCC 27380与里氏芽孢杆菌JCM 16352之间的平均核苷酸同一性和电子DNA-DNA杂交值分别为72.5%和18.5 %，均低于物种区分建议阈值（分别为96%和70 %）。菌株ATCC 27380与里氏芽孢杆菌JCM 16352之间的平均氨基酸同一性为68.72 %，高于属水平划分建议值65 %。基于表型、基因型和化学分类学数据，菌株ATCC 27380代表芽孢杆菌属中的一个新物种，为此提出名称嗜热耐旱芽孢杆菌新种（Paenibacillusxerothermodurans sp. nov.）（=DSM 520=NRRL NRS-1629=ATCC 27380）。
