Reh T A, Tully T
Dev Biol. 1986 Apr;114(2):463-9. doi: 10.1016/0012-1606(86)90210-1.
To determine whether production of new neurons of a particular type is regulated by the presence of previously differentiated neurons of the same type, we ablated all tyrosine hydroxylase immunoreactive (THIR) cells from larval frog retina with the neurotoxin 6-hydroxydopamine, and examined the retinas in subsequent weeks for newly generated THIR neurons. Three weeks after neurotoxin administration, new THIR cells appeared near the zone of neural proliferation at the ciliary margin at a higher density than that of normal retina, while the densities of other amacrine cell types, serotonin (t-HT) immunoreactive and substance P immunoreactive (SPIR), remained the same as controls. Thus the production of new retinal TRIR cells is selectively up-regulated following ablation of previously differentiated cells of this type.
为了确定特定类型新神经元的产生是否受相同类型先前分化神经元的存在调控，我们用神经毒素6-羟基多巴胺消融了幼蛙视网膜中所有酪氨酸羟化酶免疫反应性（THIR）细胞，并在随后几周检查视网膜中新生的THIR神经元。给予神经毒素三周后，新的THIR细胞出现在睫状缘神经增殖区附近，其密度高于正常视网膜，而其他无长突细胞类型，即5-羟色胺（t-HT）免疫反应性和P物质免疫反应性（SPIR）细胞的密度与对照组保持相同。因此，在消融该类型先前分化的细胞后，新的视网膜TRIR细胞的产生被选择性地上调。
