a Sussex Eye Hospital, Brighton and Sussex University Hospitals NHS Trust , Brighton , UK.
Ocul Immunol Inflamm. 2018;26(8):1189-1191. doi: 10.1080/09273948.2017.1332229. Epub 2017 Jul 12.
To describe a modified allogenic simple limbal epithelial transplant (SLET) technique with large donor tissue explants followed by keratoplasty.
A 69-year-old with conjunctival melanoma on her left eye developed total limbal stem cell deficiency (LSCD) after multiple cycles of topical mitomycin. She also had herpes stromal keratitis while on treatment with mitomycin. She underwent modified allogenic SLET with large donor limbal tissue explants glued on the cornea directly and the amniotic membrane placed over the limbal explants.
Unfortunately she had recurrence of herpes simplex keratitis that caused worsening of stromal scarring and neovascularization. She underwent penetrating keratoplasty to improve vision. There were no postoperative complications and the corneal graft remains transparent 11 months after penetrating keratoplasty with 6/12 best-corrected visual acuity.
Modified SLET with large donor limbal explants followed by keratoplasty is an effective approach to restore corneal transparency in cases of total LSCD.
描述一种改良的同种异体单纯性角膜缘上皮移植（SLET）技术，采用大供体组织片，随后进行角膜移植。
一位 69 岁的女性左眼患有结膜黑色素瘤，在多次局部应用丝裂霉素后发展为完全性角膜缘干细胞缺乏症（LSCD）。在接受丝裂霉素治疗时，她还患有疱疹性基质性角膜炎。她接受了改良的同种异体 SLET，将大供体角膜缘组织片直接粘贴在角膜上，并将羊膜置于角膜缘片上。
不幸的是，她发生了单纯疱疹性角膜炎复发，导致基质瘢痕和新生血管化加重。她接受了穿透性角膜移植以改善视力。穿透性角膜移植后 11 个月，没有术后并发症，角膜移植物仍然透明，最佳矫正视力为 6/12。
对于完全性 LSCD 患者，采用大供体角膜缘片的改良 SLET 联合角膜移植是恢复角膜透明性的有效方法。
