Thomasini Ronaldo Luis, Pereira Daniele Sirineu, Pereira Fabiana Souza Máximo, Mateo Elvis Cueva, Mota Thamires Nader, Guimarães Gabrielle Gontijo, Pereira Leani Souza Máximo, Lima Cristiano Xavier, Teixeira Mauro Martins, Teixeira Antônio Lúcio
Faculdade de Medicina de Diamantina (FAMED), NEPii - Núcleo de Estudos de Patologias Infecciosas e Inflamatórias, Universidade Federal dos Vales do Jequinhonha e Mucuri, Diamantina, Minas Gerais, Brazil.
Curso de Fisioterapia, Escola de Enfermagem, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brazil.
PLoS One. 2017 Jul 10;12(7):e0180841. doi: 10.1371/journal.pone.0180841. eCollection 2017.
Immunosenescence is an age-related reduction of immune system activity that can be associated with frailty. This study aimed to compare cytomegalovirus (CMV) and Epstein-Barr virus (EBV) reactivations (based on viremias) between young and elderly women who had a chronic CMV and/or EBV infection (i.e., an IgG+ serostatus) without an acute infection (i.e., an IgM- serostatus), and among the elderly group categorized according to frailty status. DNA was extracted from plasma using standard protocols and serostatus was determined by enzyme-linked immunosorbent assay. Quantitative real-time polymerase chain reaction analyses for CMV and EBV were carried out and viral loads were determined. Among elderly women (n = 71), 59% were positive for CMV, in contrast to only 8% of young women (n = 73). Elderly women classified as frail, pre-frail, and non-frail presented 82%, 56%, and 48% positivity for CMV, respectively. Frequency and viral load were significantly higher in the elderly group vs. the young group (p < 0.0001 and p = 0.01, respectively) and in elderly with frailty vs. those without frailty (p = 0.007 and p = 0.03, respectively). The frequency of CMV reactivation presented odds ratios of 11.77 for aging and 6.13 for frailty, and relative risks of 5.39 for aging and 1.93 for frailty. EBV was detected in 30% of the elderly women and 15% of the young women (p = 0.04); however, the viral load did not significantly differ between the two age groups. The frequency of EBV reactivation presented odds ratios of 2.36 for aging and 2.90 for frailty, and relative risks of 1.96 for aging and 2.12 for frailty. However, no difference in EBV viral load among the frailty status subgroups was found. In conclusion, the frequency of CMV reactivation was associated with aging and ongoing frailty, whereas the frequency of EBV reactivation was associated only with aging.
免疫衰老指的是与年龄相关的免疫系统活性降低，可能与身体虚弱有关。本研究旨在比较慢性巨细胞病毒（CMV）和/或EB病毒（EBV）感染（即IgG +血清学状态）且无急性感染（即IgM -血清学状态）的年轻和老年女性之间的CMV和EBV再激活情况（基于病毒血症），以及在根据虚弱状态分类的老年组中进行比较。使用标准方案从血浆中提取DNA，并通过酶联免疫吸附测定法确定血清学状态。对CMV和EBV进行定量实时聚合酶链反应分析并确定病毒载量。在老年女性（n = 71）中，59%的CMV呈阳性，相比之下年轻女性（n = 73）中只有8%呈阳性。分类为虚弱、衰弱前期和非虚弱的老年女性CMV阳性率分别为82%、56%和48%。老年组的频率和病毒载量显著高于年轻组（分别为p < 0.0001和p = 0.01），且虚弱老年组高于非虚弱老年组（分别为p = 0.007和p = 0.03）。CMV再激活频率的衰老优势比为11.77，虚弱优势比为6.13，衰老相对风险为5.39，虚弱相对风险为1.93。30%的老年女性和15%的年轻女性检测到EBV（p = 0.04）；然而，两个年龄组之间的病毒载量没有显著差异。EBV再激活频率的衰老优势比为2.36，虚弱优势比为2.90，衰老相对风险为1.96，虚弱相对风险为2.12。然而，在虚弱状态亚组之间未发现EBV病毒载量有差异。总之，CMV再激活频率与衰老和持续虚弱有关，而EBV再激活频率仅与衰老有关。
