Fu Lin, Chen Yuan-Hua, Chen Xue, Xu Shen, Yu Zhen, Xu De-Xiang
Department of Toxicology, Anhui Medical University, Hefei, China; Anhui Provincial Key Laboratory of Population Health & Aristogenics, Hefei, China.
Department of Histology and Embryology, Anhui Medical University, Hefei, China; Anhui Provincial Key Laboratory of Population Health & Aristogenics, Hefei, China.
Physiol Behav. 2017 Oct 1;179:333-339. doi: 10.1016/j.physbeh.2017.07.017. Epub 2017 Jul 9.
Vitamin D deficiency is prevalent especially in pregnant women and children. Several studies found that vitamin D status was negatively correlated with risk of senile neurobehavioral abnormality. The aim of this study was to investigate the effects of vitamin D deficiency on neurobehavioral development in mice. In vitamin D deficiency group, dams and their male pups were fed with vitamin D deficient (VDD) diet, in which vitamin D was depleted. Anxiety-related behavior, depressive-like behavior, spatial learning and memory were measured. As expected, serum 25(OH)D level was reduced in VDD diet-fed mice. An anhedonia state, a key depressive-like behavior, was observed in VDD diet-fed mice. In open-field test, peripheral time was decreased and internal time was increased in VDD diet-fed mice. In elevated plus maze, the latency of the first entry into open arms was increased and the number of crossing in open arms was elevated in VDD diet-fed mice. Morris Water Maze showed that VDD-fed mice showed longer escape latency in the first six days. On the seventh day, escape latency was increased in VDD diet-fed mice. These results provide evidence that vitamin D deficiency impairs neurobehavioral development.
维生素D缺乏症普遍存在，尤其是在孕妇和儿童中。多项研究发现，维生素D状态与老年神经行为异常风险呈负相关。本研究的目的是探讨维生素D缺乏对小鼠神经行为发育的影响。在维生素D缺乏组中，母鼠及其雄性幼崽喂食缺乏维生素D（VDD）的饮食，其中维生素D已被耗尽。测量了与焦虑相关的行为、抑郁样行为、空间学习和记忆。正如预期的那样，喂食VDD饮食的小鼠血清25（OH）D水平降低。在喂食VDD饮食的小鼠中观察到了快感缺失状态，这是一种关键的抑郁样行为。在旷场试验中，喂食VDD饮食的小鼠外周时间减少，内部时间增加。在高架十字迷宫试验中，喂食VDD饮食的小鼠首次进入开放臂的潜伏期增加，在开放臂中的穿越次数增加。莫里斯水迷宫试验表明，喂食VDD的小鼠在前六天的逃避潜伏期更长。在第七天，喂食VDD饮食的小鼠逃避潜伏期增加。这些结果提供了证据表明维生素D缺乏会损害神经行为发育。
