Department of Dermato-venereology, the Second Hospital of Shandong University, Jinan, China.
Department of Dermato-venereology, the Second Hospital of Shandong University, Jinan, China.
Biomed Pharmacother. 2017 Sep;93:713-720. doi: 10.1016/j.biopha.2017.05.127. Epub 2017 Jul 9.
Psoriasis is a common chronic dermatosis characterized by keratinocyte hyperproliferation accompanied by inflammatory reactions. Pathological changes upset the balance between keratinocyte proliferation, differentiation, and death in psoriatic lesions, suggesting that molecules with topical anti-inflammatory, anti-proliferation and anti-angiogenesis abilities may be useful for its treatment. The flavonoid astilbin is the major active component extracted from the rhizome of Smilax glabra, which has been widely used in China to treat inflammatory and autoimmune diseases. Here, we investigate the potential of astilbin as a treatment for psoriasis. We reveal that astilbin inhibits the growth of HaCaT keratinocytes. Detailed study shows that astilbin leads to S phase arrest of the cell cycle by induction of p53 and p21 and activated-AMPK. Additionally, astilbin induced keratinocyte differentiation correlated with suppression of keratin 5 (KRT5) and KRT14 proteins (the markers of epidermal basal layer) and induction KRT1 and KRT10 proteins (occurring in the upper layers). Moreover, astilbin regulates the expression of VEGF in human HaCaT keratinocytes. These results suggest that astilbin may be a promising agent for psoriasis treatment.
银屑病是一种常见的慢性皮肤病，其特征是角质形成细胞过度增殖伴有炎症反应。病理性改变扰乱了银屑病皮损中角质形成细胞增殖、分化和死亡的平衡，这表明具有局部抗炎、抗增殖和抗血管生成能力的分子可能对其治疗有用。类黄酮淫羊藿素是从菝葜根茎中提取的主要活性成分，在中国已广泛用于治疗炎症性和自身免疫性疾病。在这里，我们研究了淫羊藿素作为银屑病治疗药物的潜力。我们发现淫羊藿素可抑制 HaCaT 角质形成细胞的生长。详细研究表明，淫羊藿素通过诱导 p53 和 p21 以及激活的 AMPK 导致细胞周期 S 期停滞。此外，淫羊藿素诱导的角质形成细胞分化与抑制角蛋白 5（KRT5）和角蛋白 14（表皮基底层标志物）和诱导角蛋白 1 和角蛋白 10（发生在上层）蛋白有关。此外，淫羊藿素调节人 HaCaT 角质形成细胞中 VEGF 的表达。这些结果表明淫羊藿素可能是一种有前途的银屑病治疗药物。
