• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

过表达人肝细胞生长因子的人毛囊间充质干细胞的建立

Establishment of human hair follicle mesenchymal stem cells with overexpressed human hepatocyte growth factor.

作者信息

Zhou Dan, Cheng Hongjing, Liu Jinyu, Zhang Lei

机构信息

Department of Pediatrics, The Second Hospital of Jilin University, Changchun 130041, China.

Department of Gastroenterology, The First Hospital of Jilin University, Changchun 130041, China.

出版信息

Iran J Basic Med Sci. 2017 Jun;20(6):662-675. doi: 10.22038/IJBMS.2017.8834.

DOI:10.22038/IJBMS.2017.8834
PMID:28702144
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5501929/
Abstract

OBJECTIVES

Chronic liver disease has become a major health problem that causes serious damage to human health. Since the existing treatment effect was not ideal, we need to seek new treatment methods.

MATERIALS AND METHODS

We utilized the gene recombination technology to obtain the human hair mesenchymal stem cells which overexpression of human hepatocyte growth factor (hHGF). Furthermore, we verified the property of transfected cells through detecting surface marker by flow cytometry.

RESULTS

We show here establishment of the hHGF-overexpressing lentivirus vector, and successfully transfection to human hair follicle mesenchymal stem cells. The verified experiments could demonstrate the human hair follicle mesenchymal stem cells which have been transfected still have the properties of stem cells.

CONCLUSION

We successfully constructed human hair follicle mesenchymal stem cells which overexpression hHGF, and maintain the same properties compared with pro-transfected cells.

摘要

目的

慢性肝病已成为严重损害人类健康的主要健康问题。由于现有治疗效果不理想，我们需要寻找新的治疗方法。

材料与方法

我们利用基因重组技术获得过表达人肝细胞生长因子（hHGF）的人毛囊间充质干细胞。此外，我们通过流式细胞术检测表面标志物来验证转染细胞的特性。

结果

我们在此展示了hHGF过表达慢病毒载体的构建，并成功转染到人毛囊间充质干细胞。验证实验可以证明已转染的人毛囊间充质干细胞仍具有干细胞特性。

结论

我们成功构建了过表达hHGF的人毛囊间充质干细胞，且与转染前细胞相比保持相同特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/c2cce7d70b3c/IJBMS-20-662-g020.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/4dc80d831adc/IJBMS-20-662-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/d5eee2d0a239/IJBMS-20-662-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/20784005fe04/IJBMS-20-662-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/eb7b3ff09747/IJBMS-20-662-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/a7da1e15767e/IJBMS-20-662-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/22a07cebdad8/IJBMS-20-662-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/bc1af718577e/IJBMS-20-662-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/2cd96cafcbd1/IJBMS-20-662-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/1df3603b01e9/IJBMS-20-662-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/a75861c68493/IJBMS-20-662-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/7db2bf93601c/IJBMS-20-662-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/7fd754b910f0/IJBMS-20-662-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/c21efc4eebe2/IJBMS-20-662-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/c784e70530b2/IJBMS-20-662-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/3f7429d9695b/IJBMS-20-662-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/5ed3fed6a778/IJBMS-20-662-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/6cb02aaba868/IJBMS-20-662-g017.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/4f3faede7a8a/IJBMS-20-662-g018.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/ab7f58c1188f/IJBMS-20-662-g019.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/c2cce7d70b3c/IJBMS-20-662-g020.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/4dc80d831adc/IJBMS-20-662-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/d5eee2d0a239/IJBMS-20-662-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/20784005fe04/IJBMS-20-662-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/eb7b3ff09747/IJBMS-20-662-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/a7da1e15767e/IJBMS-20-662-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/22a07cebdad8/IJBMS-20-662-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/bc1af718577e/IJBMS-20-662-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/2cd96cafcbd1/IJBMS-20-662-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/1df3603b01e9/IJBMS-20-662-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/a75861c68493/IJBMS-20-662-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/7db2bf93601c/IJBMS-20-662-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/7fd754b910f0/IJBMS-20-662-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/c21efc4eebe2/IJBMS-20-662-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/c784e70530b2/IJBMS-20-662-g014.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/3f7429d9695b/IJBMS-20-662-g015.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/5ed3fed6a778/IJBMS-20-662-g016.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/6cb02aaba868/IJBMS-20-662-g017.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/4f3faede7a8a/IJBMS-20-662-g018.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/ab7f58c1188f/IJBMS-20-662-g019.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/76c8/5501929/c2cce7d70b3c/IJBMS-20-662-g020.jpg

相似文献

1
Establishment of human hair follicle mesenchymal stem cells with overexpressed human hepatocyte growth factor.过表达人肝细胞生长因子的人毛囊间充质干细胞的建立
Iran J Basic Med Sci. 2017 Jun;20(6):662-675. doi: 10.22038/IJBMS.2017.8834.
2
Therapeutic role of human hepatocyte growth factor (HGF) in treating hair loss.人肝细胞生长因子（HGF）在治疗脱发中的治疗作用。
PeerJ. 2016 Nov 1;4:e2624. doi: 10.7717/peerj.2624. eCollection 2016.
3
[Research of lentiviral vector mediated growth factor gene-modified bone marrow mesenchymal stem cells].慢病毒载体介导生长因子基因修饰骨髓间充质干细胞的研究
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2010 Aug;24(8):972-6.
4
Therapeutic effects of hepatocyte growth factor-overexpressing human umbilical cord blood-derived mesenchymal stem cells on liver fibrosis in rats.人脐带血间充质干细胞过表达肝细胞生长因子对大鼠肝纤维化的治疗作用。
Cell Biol Int. 2014 Jan;38(1):106-16. doi: 10.1002/cbin.10186. Epub 2013 Oct 17.
5
[Transplantation of mesenchymal stem cells transfected with hepatocyte growth factor gene improves heart function in rats with heart failure].肝细胞生长因子基因转染间充质干细胞移植改善心力衰竭大鼠心脏功能
Zhongguo Ying Yong Sheng Li Xue Za Zhi. 2009 Nov;25(4):521-6.
6
Effects of hepatocyte growth factor gene-transfected mesenchymal stem cells on dimethylnitrosamine-induced liver fibrosis in rats.肝细胞生长因子基因转染的间充质干细胞对二甲基亚硝胺诱导的大鼠肝纤维化的影响。
Growth Factors. 2019 Aug;37(3-4):105-119. doi: 10.1080/08977194.2019.1652399. Epub 2019 Aug 27.
7
[Construction of Lentivirus Vector Carrying HGF and Exploration of Transfection Condition of Rat Adipocyte-derived Mesenchymal Stem Cells].[携带肝细胞生长因子的慢病毒载体构建及大鼠脂肪来源间充质干细胞转染条件探索]
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2015 Apr;23(2):490-4. doi: 10.7534/j.issn.1009-2137.2015.02.038.
8
Human amniotic mesenchymal stem cells improve ovarian function in natural aging through secreting hepatocyte growth factor and epidermal growth factor.人羊膜间充质干细胞通过分泌肝细胞生长因子和表皮生长因子改善自然衰老过程中的卵巢功能。
Stem Cell Res Ther. 2018 Mar 9;9(1):55. doi: 10.1186/s13287-018-0781-9.
9
Isolation and characterization of culture of hair follicle cells differentiated from umbilical cord blood mesenchymal stem cells.从脐带血间充质干细胞分化而来的毛囊细胞培养物的分离与鉴定
Exp Ther Med. 2017 Jul;14(1):303-307. doi: 10.3892/etm.2017.4456. Epub 2017 May 16.
10
Transplantation of adipose-derived stem cells overexpressing hHGF into cardiac tissue.将过表达人肝细胞生长因子的脂肪来源干细胞移植到心脏组织中。
Biochem Biophys Res Commun. 2009 Feb 20;379(4):1084-90. doi: 10.1016/j.bbrc.2009.01.019. Epub 2009 Jan 13.

引用本文的文献

1
Recent advances in the therapeutic efficacy of hepatocyte growth factor gene-modified mesenchymal stem cells in multiple disease settings.肝细胞生长因子基因修饰的间充质干细胞在多种疾病环境中的治疗效果研究进展
J Cell Mol Med. 2022 Sep;26(18):4745-4755. doi: 10.1111/jcmm.17497. Epub 2022 Aug 3.
2
Extracellular vesicles from hair follicle-derived mesenchymal stromal cells: isolation, characterization and therapeutic potential for chronic wound healing.毛囊来源的间充质基质细胞外囊泡：分离、鉴定及其在慢性伤口愈合治疗中的潜能。
Stem Cell Res Ther. 2022 Apr 8;13(1):147. doi: 10.1186/s13287-022-02824-0.
3
Human hair follicle-derived mesenchymal stem cells: Isolation, expansion, and differentiation.

本文引用的文献

1
An update on liver transplantation: A critical review.肝移植的最新进展：批判性回顾。
J Autoimmun. 2016 Jan;66:51-9. doi: 10.1016/j.jaut.2015.08.021. Epub 2015 Sep 5.
2
New Tools in Experimental Cellular Therapy for the Treatment of Liver Diseases.用于治疗肝脏疾病的实验性细胞疗法中的新工具
Curr Transplant Rep. 2015 Jun 1;2(2):202-210. doi: 10.1007/s40472-015-0059-4.
3
Experimental hepatocyte xenotransplantation--a comprehensive review of the literature.实验性肝细胞异种移植——文献综述
人毛囊来源的间充质干细胞：分离、扩增及分化
World J Stem Cells. 2020 Jun 26;12(6):462-470. doi: 10.4252/wjsc.v12.i6.462.
4
Adipose tissue stem cells in regenerative medicine.再生医学中的脂肪组织干细胞。
Ecancermedicalscience. 2018 Mar 28;12:822. doi: 10.3332/ecancer.2018.822. eCollection 2018.
Xenotransplantation. 2015 Jul-Aug;22(4):239-48. doi: 10.1111/xen.12170. Epub 2015 May 7.
4
Mutant MMP-9 and HGF gene transfer enhance resolution of CCl4-induced liver fibrosis in rats: role of ASH1 and EZH2 methyltransferases repression.突变型基质金属蛋白酶-9和肝细胞生长因子基因转移可增强四氯化碳诱导的大鼠肝纤维化的消退：ASH1和EZH2甲基转移酶抑制的作用
PLoS One. 2014 Nov 7;9(11):e112384. doi: 10.1371/journal.pone.0112384. eCollection 2014.
5
Therapeutic effect of hepatocyte growth factor-secreting mesenchymal stem cells in a rat model of liver fibrosis.分泌肝细胞生长因子的间充质干细胞在大鼠肝纤维化模型中的治疗作用
Exp Mol Med. 2014 Aug 22;46(8):e110. doi: 10.1038/emm.2014.49.
6
Cell and tissue engineering for liver disease.用于肝病的细胞与组织工程
Sci Transl Med. 2014 Jul 16;6(245):245sr2. doi: 10.1126/scitranslmed.3005975.
7
Donor transmitted and de novo cancer after liver transplantation.肝移植后供体传播性和新发癌症
World J Gastroenterol. 2014 May 28;20(20):6170-9. doi: 10.3748/wjg.v20.i20.6170.
8
Bioreactor technologies to support liver function in vitro.支持体外肝功能的生物反应器技术。
Adv Drug Deliv Rev. 2014 Apr;69-70:132-57. doi: 10.1016/j.addr.2014.02.011. Epub 2014 Mar 5.
9
Therapeutic effects of hepatocyte growth factor-overexpressing human umbilical cord blood-derived mesenchymal stem cells on liver fibrosis in rats.人脐带血间充质干细胞过表达肝细胞生长因子对大鼠肝纤维化的治疗作用。
Cell Biol Int. 2014 Jan;38(1):106-16. doi: 10.1002/cbin.10186. Epub 2013 Oct 17.
10
Hepatocyte growth factor twenty years on: Much more than a growth factor.肝细胞生长因子二十年的研究进展：远不止一种生长因子。
J Gastroenterol Hepatol. 2011 Jan;26 Suppl 1:188-202. doi: 10.1111/j.1440-1746.2010.06549.x.