Wang Bo, Liu Xiao-Mei, Liu Zi-Nan, Wang Yuan, Han Xing, Lian Ao-Bo, Mu Ying, Jin Ming-Hua, Liu Jin-Yu
Department of Toxicology, School of Public Health, Jilin University, Changchun 130021, Jilin Province, China.
Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310000, Zhejiang Province, China.
World J Stem Cells. 2020 Jun 26;12(6):462-470. doi: 10.4252/wjsc.v12.i6.462.
Hair follicles are easily accessible skin appendages that protect against cold and potential injuries. Hair follicles contain various pools of stem cells, such as epithelial, melanocyte, and mesenchymal stem cells (MSCs) that continuously self-renew, differentiate, regulate hair growth, and maintain skin homeostasis. Recently, MSCs derived from the dermal papilla or dermal sheath of the human hair follicle have received attention because of their accessibility and broad differentiation potential. In this review, we describe the applications of human hair follicle-derived MSCs (hHF-MSCs) in tissue engineering and regenerative medicine. We have described protocols for isolating hHF-MSCs from human hair follicles and their culture condition in detail. We also summarize strategies for maintaining hHF-MSCs in a highly proliferative but undifferentiated state after repeated passages, including supplementation of growth factors, 3D suspension culture technology, and 3D aggregates of MSCs. In addition, we report the potential of hHF-MSCs in obtaining induced smooth muscle cells and tissue-engineered blood vessels, regenerated hair follicles, induced red blood cells, and induced pluripotent stem cells. In summary, the abundance, convenient accessibility, and broad differentiation potential make hHF-MSCs an ideal seed cell source of regenerative medical and cell therapy.
毛囊是易于获取的皮肤附属器，可抵御寒冷和潜在损伤。毛囊包含各种干细胞库，如上皮干细胞、黑素细胞干细胞和间充质干细胞（MSCs），这些干细胞可不断自我更新、分化、调节头发生长并维持皮肤稳态。最近，源自人毛囊真皮乳头或真皮鞘的间充质干细胞因其易于获取和广泛的分化潜能而受到关注。在本综述中，我们描述了人毛囊来源的间充质干细胞（hHF-MSCs）在组织工程和再生医学中的应用。我们详细描述了从人毛囊中分离hHF-MSCs的方案及其培养条件。我们还总结了在反复传代后将hHF-MSCs维持在高增殖但未分化状态的策略，包括补充生长因子、3D悬浮培养技术和间充质干细胞的3D聚集体。此外，我们报道了hHF-MSCs在获得诱导平滑肌细胞和组织工程血管、再生毛囊、诱导红细胞和诱导多能干细胞方面的潜力。总之，hHF-MSCs丰富、易于获取且具有广泛的分化潜能，使其成为再生医学和细胞治疗的理想种子细胞来源。
