Chen P M, Chiu C F, Chiou T J, Tzeng C H, Lin S T, Wang L S, Lee Y, Chien K Y, Chang S S, Maeda S
Leuk Res. 1986;10(4):369-73. doi: 10.1016/0145-2126(86)90065-2.
In the present study an immunofluorescence using KH-2 cells as target cells, has been developed for the screening of 1200 serum samples from normal individuals and 450 of cases from patients with various malignancies. The positive anti-HTLV-I antibody rate in the former group is 0.083% (1/1200) and while in the latter it is found to be 1.8% (8/450) (including 3 adult T-cell leukemia/lymphoma cases of the 92 hematopoietic and 5 of 358 non-hematopoietic malignancies). The differences between the two groups are found to be significantly different (p value is less than 0.0001). In addition to the 3 adult T-cell leukemia/lymphoma cases, the 5 seropositive cancer patients are of 5 different diseases. We have searched for the adult T-cell leukemia virus antigen and the p19 core protein in lymphoid cells of seropositive persons and the only positive cases were from cells of two proven adult T-cell leukemia (ATL) patients. Our results suggest that Taiwan is not an endemic area of adult T-cell leukemia virus and that KH-2 cells may be used for the detection of anti-HTLV-I antibodies.
在本研究中，已开发出一种以KH - 2细胞为靶细胞的免疫荧光法，用于筛查1200份正常个体的血清样本和450份各类恶性肿瘤患者的样本。前一组中抗HTLV - I抗体阳性率为0.083%（1/1200），而后一组中该阳性率为1.8%（8/450）（包括92例造血系统恶性肿瘤中的3例成人T细胞白血病/淋巴瘤病例以及358例非造血系统恶性肿瘤中的5例）。发现两组之间存在显著差异（p值小于0.0001）。除了3例成人T细胞白血病/淋巴瘤病例外，5例血清学阳性的癌症患者患有5种不同疾病。我们在血清学阳性者的淋巴细胞中搜索了成人T细胞白血病病毒抗原和p19核心蛋白，仅有的阳性病例来自两名经证实的成人T细胞白血病（ATL）患者的细胞。我们的结果表明台湾不是成人T细胞白血病病毒的流行地区，且KH - 2细胞可用于检测抗HTLV - I抗体。
