Antoniades H N, Scher C D, Stiles C D
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1809-13. doi: 10.1073/pnas.76.4.1809.
Human platelets contain a polypeptide growth factor that stimulates the proliferation of connective tissue cells. Purification of this platelet-derived growth factor (PDGF) was accomplished by heat (100 degrees C) treatment of washed platelets and subsequent ion-exchange chromatography, gel filtration in 1 M acetic acid, isoelectric focusing, and preparative sodium dodecyl sulfate/polyacrylamide gel electrophoresis. PDGF has an isoelectric point of 9.8 and a molecular weight ranging from 13,000 to 16,000 as judged by gel filtration in 1 M acetic acid or analytical sodium dodecyl sulfate gel electrophoresis under reducing conditions. The specific activity of the purified PDGF is 20 million times greater than that found in unfractionated human serum. Purified PDGF stimulates replicative DNA synthesis and cell proliferation in quiescent density-arrested cultures of BALB/c 3T3 cells at concentrations of 1 ng/ml (0.1 nM).
人类血小板含有一种能刺激结缔组织细胞增殖的多肽生长因子。通过对洗涤过的血小板进行加热（100摄氏度）处理，随后进行离子交换色谱、在1 M乙酸中进行凝胶过滤、等电聚焦以及制备性十二烷基硫酸钠/聚丙烯酰胺凝胶电泳，实现了这种血小板衍生生长因子（PDGF）的纯化。PDGF的等电点为9.8，在1 M乙酸中进行凝胶过滤或在还原条件下进行分析性十二烷基硫酸钠凝胶电泳判断，其分子量在13,000至16,000之间。纯化后的PDGF的比活性比未分级的人血清中的比活性高2000万倍。纯化后的PDGF在浓度为1 ng/ml（0.1 nM）时，能刺激静止的、密度抑制的BALB/c 3T3细胞培养物中的复制性DNA合成和细胞增殖。
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