Department of Oral Biology, School of Dentistry, Medical University of Vienna, Sensengasse 2a, 1090, Vienna, Austria.
Department of Conservative Dentistry, School of Dentistry, Universidad de Chile, Av. Sergio Livingstone, 943, Santiago, Chile.
Clin Oral Investig. 2020 Feb;24(2):569-584. doi: 10.1007/s00784-019-03156-9. Epub 2019 Dec 26.
To systematically assess the effects of platelet-rich fibrin (PRF) on in vitro cellular behavior.
A systematic electronic search using MEDLINE database was performed. In vitro studies using PRF were considered and articles published up to June 31, 2018 were screened. Eligible studies were selected based on the use of human PRF.
In total, 1746 titles were identified with the search terms, from these 37 met the inclusion criteria and were chosen for data extraction. In addition, 16 new studies, mainly published in 2019, were also included in the analysis resulting in 53 studies. No meta-analysis could be performed due to the heterogeneity of study designs. Included studies show that PRF enhances proliferation, migration, adhesion, and osteogenic differentiation on a variety of cell types along with cell signaling activation. Furthermore, PRF reduces inflammation, suppresses osteoclastogenesis, and increases the expression of various growth factors in mesenchymal cells. Despite some notable differences of the studies, the overall findings suggest a positive effect of PRF on cell proliferation, migration, adhesion, differentiation, and inflammation pointing towards a therapeutic potential in regenerative dentistry.
PRF serves as a reservoir of bioactive molecules to support wound healing and bone regeneration. Although the cellular mechanisms by which PRF supports the clinical outcomes remain unclear, in vitro research provides possible explanations. This systematic review aims to provide an update of the existing research on how PRF affects basic physiological processes in vitro. The overall findings suggest that PRF induces cell proliferation, migration, adhesion, and differentiation along with possessing anti-inflammatory properties further supporting its therapeutic potential in wound healing and bone regeneration.
系统评估富血小板纤维蛋白（PRF）对体外细胞行为的影响。
使用 MEDLINE 数据库进行系统电子检索。考虑使用 PRF 的体外研究，并筛选截至 2018 年 6 月 31 日发表的文章。根据使用人 PRF，选择符合条件的研究。
共确定了 1746 个标题的搜索词，其中 37 个符合纳入标准并选择用于数据提取。此外，还纳入了 16 项主要在 2019 年发表的新研究，分析中总计纳入了 53 项研究。由于研究设计的异质性，无法进行荟萃分析。纳入的研究表明，PRF 增强了多种细胞类型的增殖、迁移、黏附和成骨分化，同时激活细胞信号转导。此外，PRF 减少炎症、抑制破骨细胞生成，并增加间充质细胞中各种生长因子的表达。尽管研究存在一些明显差异，但总体研究结果表明 PRF 对细胞增殖、迁移、黏附、分化和炎症有积极影响，提示其在再生牙科领域具有治疗潜力。
PRF 作为生物活性分子的储存库，支持伤口愈合和骨再生。尽管 PRF 支持临床结果的细胞机制尚不清楚，但体外研究提供了可能的解释。本系统综述旨在提供关于 PRF 如何影响体外基本生理过程的现有研究的最新信息。总体研究结果表明，PRF 诱导细胞增殖、迁移、黏附和分化，同时具有抗炎特性，进一步支持其在伤口愈合和骨再生中的治疗潜力。
