Nishikawa Yuko, Morishita Seita, Horie Taeko, Fukumoto Masanori, Sato Takaki, Kida Teruyo, Oku Hidehiro, Sugasawa Jun, Ikeda Tsunehiko, Nakamura Kimitoshi
Department of Ophthalmology, Osaka Medical College, Takatsuki-City, Osaka, Japan.
Nakamura Eye Clinic, Matsumoto-City, Nagano, Japan.
PLoS One. 2017 Jul 13;12(7):e0180933. doi: 10.1371/journal.pone.0180933. eCollection 2017.
The purpose of this study was to compare steroid hormone concentration levels in the vitreous and serum of vitreoretinal disease patients to elucidate the possibility of neurosteroid production in the retina. Serum and vitreous samples were collected from vitrectomy patients, and estradiol (E2) and testosterone (T) concentrations were measured using electro-chemiluminescence immunoassay. We measured E2 in epiretinal membrane (ERM, n = 14), macular hole (MH, n = 18), proliferative diabetic retinopathy (PDR, n = 20), and retinal detachment (RD, n = 19) cases, and T in ERM (n = 14), MH (n = 17), PDR (n = 13), and RD (n = 17) cases. No statistically significant age differences existed among the groups. Mean respective E2 concentrations (pg/ml) in the male/female vitreous were ERM: 6.67±4.04/18.82±7.10, MH: 10.3±7.02/17.00±4.8, PDR: 4.2±3.05/15.83±3.46, and RD: 10.00±4.58/16.06±4.57, while those in serum were ERM: 31.67±5.51/5.82±1.08, MH: 21.00±8.89/7.53±3.2, PDR: 29.20±7.07/12.75±10.62, and RD: 24.33±6.51/7.5±4.42. E2 concentrations were significantly higher (P<0.001) in the male serum than vitreous, yet significantly higher in the female vitreous than serum. Mean respective T concentrations (ng/ml) in the male/female vitreous were ERM: 0.15±0.03/0.15±0.01, MH: 0.15±0.01/0.15±0.01, PDR: 0.15±0.03/0.16±0.12, and RD: 0.14±0.01/0.17±0.08, while those in serum were ERM: 4.54±1.46/0.16±0.01, MH: 8.04±2.29/0.16±0.10, PDR: 5.14±1.54/0.22±0.11, and RD: 3.24±0.75/0.17±0.10. T concentrations were high in the male serum, yet extremely low in the male and female vitreous and female serum. High concentrations of E2 were found in the vitreous, and women, in particular, exhibited significantly higher concentrations in the vitreous than in the serum. This finding suggests the possibility that in vitreoretinal disease cases, the synthesis of E2 is increased locally only in female eyes.
本研究的目的是比较玻璃体视网膜疾病患者玻璃体和血清中的类固醇激素浓度水平，以阐明视网膜中神经甾体产生的可能性。从玻璃体切除患者中采集血清和玻璃体样本，并使用电化学发光免疫分析法测量雌二醇（E2）和睾酮（T）的浓度。我们测量了视网膜前膜（ERM，n = 14）、黄斑裂孔（MH，n = 18）、增殖性糖尿病视网膜病变（PDR，n = 20）和视网膜脱离（RD，n = 19）病例中的E2，以及ERM（n = 14）、MH（n = 17）、PDR（n = 13）和RD（n = 17）病例中的T。各组之间不存在统计学上显著的年龄差异。男性/女性玻璃体中各自的平均E2浓度（pg/ml）分别为：ERM：6.67±4.04/18.82±7.10，MH：10.3±7.02/17.00±4.8，PDR：4.2±3.05/15.83±3.46，RD：10.00±4.58/16.06±4.57，而血清中的浓度分别为：ERM：31.67±5.51/5.82±1.08，MH：21.00±8.89/7.53±3.2，PDR：29.20±7.07/12.75±10.62，RD：24.33±6.51/7.5±4.42。男性血清中的E2浓度显著高于玻璃体（P<0.001），而女性玻璃体中的E2浓度显著高于血清。男性/女性玻璃体中各自的平均T浓度（ng/ml）分别为：ERM：0.15±0.03/0.15±0.01，MH：0.15±0.01/0.15±0.01，PDR：0.15±0.03/0.16±0.12，RD：0.14±0.01/0.17±0.08，而血清中的浓度分别为：ERM：4.54±1.46/0.16±0.01，MH：8.04±2.29/0.16±0.10，PDR：5.14±1.54/0.22±0.11，RD：3.24±0.75/0.17±0.10。男性血清中T浓度较高，但男性和女性玻璃体以及女性血清中T浓度极低。玻璃体中发现高浓度的E2，尤其是女性，其玻璃体中的浓度显著高于血清。这一发现提示在玻璃体视网膜疾病病例中，仅在女性眼中局部E2合成增加的可能性。
