用β-环糊精接枝柠檬酸盐包覆的银纳米颗粒比色法检测核黄素
Colorimetric detection of riboflavin by silver nanoparticles capped with β-cyclodextrin-grafted citrate.
作者信息
Ma Qi, Song Jinping, Zhang Sufang, Wang Meifang, Guo Yong, Dong Chuan
机构信息
College of Chemistry and Environmental Engineering, and Institute of Applied Chemistry, Shanxi Datong University, Datong, Shanxi 037009, China.
College of Chemistry and Environmental Engineering, and Institute of Applied Chemistry, Shanxi Datong University, Datong, Shanxi 037009, China; Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
出版信息
Colloids Surf B Biointerfaces. 2016 Dec 1;148:66-72. doi: 10.1016/j.colsurfb.2016.08.040. Epub 2016 Aug 24.
β-Cyclodextrin-grafted citrate was used for the first time as a stabilizer and reducer to prepare silver nanoparticles (AgNPs). The as-synthesized AgNPs were further characterized by UV-vis absorption spectroscopy, powder X-ray diffraction spectroscopy, and transmission electron microscopy. The results show that the presence of riboflavin caused severe aggregation of the nanoparticles, thereby inducing a colour change from yellow to red. H NMR further verified the formation of non-inclusion complexes between riboflavin and β-cyclodextrin-grafted citrate. Hydrogen bond was considered the main driving force of the interaction between the riboflavin and external rim of β-cyclodextrin. Based on these observations, the as-synthesized AgNPs were utilized to develop a novel colorimetric sensor for riboflavin detection. This colorimetric probe showed excellent selectivity and high sensitivity for riboflavin with a detection limit of 167nM.
首次使用β-环糊精接枝柠檬酸盐作为稳定剂和还原剂来制备银纳米颗粒（AgNPs）。通过紫外可见吸收光谱、粉末X射线衍射光谱和透射电子显微镜对合成的AgNPs进行了进一步表征。结果表明，核黄素的存在导致纳米颗粒严重聚集，从而引起颜色从黄色变为红色。1H NMR进一步证实了核黄素与β-环糊精接枝柠檬酸盐之间形成了非包合物。氢键被认为是核黄素与β-环糊精外缘之间相互作用的主要驱动力。基于这些观察结果，合成的AgNPs被用于开发一种用于检测核黄素的新型比色传感器。这种比色探针对核黄素表现出优异的选择性和高灵敏度，检测限为167nM。
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