Horecka Anna, Biernacka Jadwiga, Hordyjewska Anna, Dąbrowski Wojciech, Terlecki Piotr, Zubilewicz Tomasz, Musik Irena, Kurzepa Jacek
1 Department of Medical Chemistry, Medical University of Lublin, Lublin, Poland.
2 I Clinic of Anaesthesiology and Intensive Therapy with Clinical Paediatric Department, Medical University of Lublin, Lublin, Poland.
Phlebology. 2018 Aug;33(7):464-469. doi: 10.1177/0268355517721055. Epub 2017 Jul 14.
Objective Our objective was to evaluate the state of oxidative stress in the great saphenous varicose vein wall and blood of varicose vein patients taken from the antecubital vein. Methods The superoxide dismutase, reduced glutathione (GSH) and total antioxidant status were measured with commercially available colorimetric kits in erythrocytes, plasma and varicose vein wall of 65 patients (second degree of clinical state classification, etiology, anatomy and pathophysiology) aged 22-70 (49 women, 16 men) in comparison to normal great saphenous vein walls collected from 10 patients who underwent coronary artery bypass graft and blood collected from 20 healthy individuals. Results A statistically significant decrease (p < 0.001) in superoxide dismutase activity in erythrocytes and the increase (p < 0.05) in superoxide dismutase activity in varicose vein has been observed. There have been no significant changes in the concentration of GSH in plasma and in varicose vein. The decreased concentration of total antioxidant status in plasma (p < 0.001) and in varicose vein wall (p < 0.05) in comparison to the control has been noticed. Conclusion The varicose vein patients are affected by oxidative stress. Our results indicate impaired antioxidant defense mechanism in the blood of varicose vein patients. In contrast to the blood, an increased process of antioxidant defense in the varicose vein wall was noticed.
目的 我们的目的是评估取自肘前静脉的大隐静脉曲张患者大隐静脉壁和血液中的氧化应激状态。方法 采用市售比色试剂盒，对65例年龄在22 - 70岁（49名女性，16名男性）的患者（临床状态分类、病因、解剖和病理生理学为二级）的红细胞、血浆和曲张静脉壁中的超氧化物歧化酶、还原型谷胱甘肽（GSH）和总抗氧化状态进行测量，并与10例接受冠状动脉旁路移植术患者的正常大隐静脉壁以及20名健康个体采集的血液进行比较。结果 观察到红细胞中超氧化物歧化酶活性有统计学显著下降（p < 0.001），曲张静脉中超氧化物歧化酶活性增加（p < 0.05）。血浆和曲张静脉中GSH浓度无显著变化。与对照组相比，血浆（p < 0.001）和曲张静脉壁（p < 0.05）中总抗氧化状态浓度降低。结论 大隐静脉曲张患者受到氧化应激影响。我们的结果表明大隐静脉曲张患者血液中的抗氧化防御机制受损。与血液相反，在曲张静脉壁中观察到抗氧化防御过程增强。
