Gwozdzinski Lukasz, Bernasinska-Slomczewska Joanna, Hikisz Pawel, Wiktorowska-Owczarek Anna, Kowalczyk Edward, Pieniazek Anna
Department of Pharmacology and Toxicology, Medical University of Lodz, 90-752 Lodz, Poland.
Department of Oncobiology and Epigenetics, Faculty of Biology and Environmental Protection, University of Lodz, 90-236 Lodz, Poland.
Biomedicines. 2023 Jun 13;11(6):1702. doi: 10.3390/biomedicines11061702.
In this study, we investigated the properties of human varicose vein (VV) endothelial cells (HVVEC) in comparison to the human umbilical vein endothelial cells (HUVEC). The cells were treated with three bioactive compounds with proven beneficial effects in the therapy of patients with VV, diosmin, escin, and bromelain. Two concentrations of tested drugs were used (1, 10 mg/mL), which did not affect the viability of either cell type. Escin led to a slight generation of reactive oxygen species in HUVEC cells. We observed a slight release of superoxide in HVVEC cells upon treatment with diosmin and escin. Diosmin and bromelain showed a tendency to release nitric oxide in HUVEC. Using membrane fluorescent probes, we demonstrated a reduced fluidity of HVVEC, which may lead to their increased adhesion, and, consequently, a much more frequent occurrence of venous thrombosis. For the first time, we show the mechanism of action of drugs used in VV therapy on endothelial cells derived from a VV. Studies with HVVEC have shown that tested drugs may lead to a reduction in the adhesive properties of these cells, and thus to a lower risk of thrombosis.
在本研究中，我们将人静脉曲张（VV）内皮细胞（HVVEC）与人脐静脉内皮细胞（HUVEC）进行比较，研究了HVVEC的特性。用三种已证实对VV患者治疗有有益作用的生物活性化合物——地奥司明、七叶皂苷和菠萝蛋白酶处理细胞。使用了两种浓度的受试药物（1、10mg/mL），这两种浓度均不影响任何一种细胞类型的活力。七叶皂苷导致HUVEC细胞中轻微产生活性氧。在用地奥司明和七叶皂苷处理后，我们观察到HVVEC细胞中有轻微的超氧化物释放。地奥司明和菠萝蛋白酶显示出在HUVEC中释放一氧化氮的趋势。使用膜荧光探针，我们证明了HVVEC的流动性降低，这可能导致其黏附增加，进而导致静脉血栓形成的频率更高。我们首次展示了VV治疗中使用的药物对源自VV的内皮细胞的作用机制。对HVVEC的研究表明，受试药物可能导致这些细胞的黏附特性降低，从而降低血栓形成的风险。
