Sunagawa Keishin, Hishima Tsunekazu, Fukumoto Hitomi, Hasegawa Hideki, Katano Harutaka
Department of Pathology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan.
Department of Pathology, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142-8555, Japan.
BMC Res Notes. 2017 Jul 14;10(1):279. doi: 10.1186/s13104-017-2603-z.
Epstein-Barr virus (EBV) encodes at least 25 pri-microRNAs (miRNAs) in two regions of its DNA genome, BART and BHRF. B95-8, an EBV reference strain, has a deletion in the BART region. However, no information is available on the deletions or mutations in the BART and BHRF regions in clinical samples of EBV-associated lymphoma.
Nine DNA fragments encoding miR-BARTs and two coding miR-BHRF1s were amplified by PCR from DNA samples extracted from 16 cases of EBV-associated lymphoma. All the PCR products were sequenced directly. DNA fragments encoding miR-BARTs and miR-BHRF1-1 were successfully amplified from all samples. An adenine-to-guanine mutation in the DNA fragment encoding miR-BART2-3p was detected in four of the 16 cases, and a cytosine-to-thymidine mutation in the DNA fragment encoding miR-BART11-3p was detected in one of the 16 samples. These mutations were not associated with any histological categories of lymphoma. In conclusion, mutations were rarely observed in the DNA encoding viral miRNAs in cases of lymphoma. This suggests that the DNA sequences of EBV-encoded miR-BARTs and miR-BHRF1-1 are conserved in EBV-associated lymphoma.
爱泼斯坦-巴尔病毒（EBV）在其DNA基因组的两个区域BART和BHRF中编码至少25种初级微小RNA（miRNA）。EBV参考菌株B95-8在BART区域存在缺失。然而，关于EBV相关淋巴瘤临床样本中BART和BHRF区域的缺失或突变情况尚无相关信息。
通过PCR从16例EBV相关淋巴瘤患者的DNA样本中扩增出9个编码miR-BART的DNA片段和2个编码miR-BHRF1的片段。所有PCR产物均直接进行测序。在所有样本中均成功扩增出编码miR-BART和miR-BHRF1-1的DNA片段。在16例中的4例中检测到编码miR-BART2-3p的DNA片段存在腺嘌呤到鸟嘌呤的突变，在16个样本中的1例中检测到编码miR-BART11-3p的DNA片段存在胞嘧啶到胸腺嘧啶的突变。这些突变与淋巴瘤的任何组织学类型均无关联。总之，在淋巴瘤病例中，病毒miRNA编码DNA中很少观察到突变。这表明EBV编码的miR-BART和miR-BHRF1-1的DNA序列在EBV相关淋巴瘤中是保守的。
