Bonner W M, Stedman J D
Proc Natl Acad Sci U S A. 1979 May;76(5):2190-4. doi: 10.1073/pnas.76.5.2190.
Water-soluble carbodiimide crosslinks histones 1A and 1B to histone 2A and to semi-histone A24 in chromatin from mouse cells. The identities of the histone species present in the crosslinked dimers were determined by fingerprinting. The molar ratio of H1--A24 to H2A is the same as the molar ratio of A24 to H2A in these cells. The H1-H2A crosslinks form equally well in whole nuclei, lysed nuclei, and H1-containing mononucleosomes isolated from a sucrose gradient. These results suggest that there exist major H1 interactions within the nucleosome.
水溶性碳二亚胺将小鼠细胞染色质中的组蛋白1A和1B与组蛋白2A以及半组蛋白A24交联。通过指纹图谱确定了交联二聚体中存在的组蛋白种类的身份。在这些细胞中，H1-A24与H2A的摩尔比与A24与H2A的摩尔比相同。H1-H2A交联在完整细胞核、裂解细胞核以及从蔗糖梯度中分离出的含H1单核小体中形成的效果相同。这些结果表明核小体内存在主要的H1相互作用。
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