Płytycz B, Pedersen A, Bøgwald J, Seljelid R
Virchows Arch B Cell Pathol Incl Mol Pathol. 1986;50(3):285-92. doi: 10.1007/BF02889907.
An ascites subline (AA) of the murine sarcoma MC1M grows in vivo in the peritoneal cavity but dies in vitro when cultured on glass or collagen. The viability of AA cells in vitro is not influenced in cocultures with fibroblast cell line L929, and is diminished in cocultures supplemented with macrophage culture supernatant or in cocultures with non-adherent peritoneal cells. However, AA cells proliferate in vitro on glass or collagen when cocultured with syngeneic, semisyngeneic, and allogeneic peritoneal macrophages. This was demonstrated by tritiated thymidine incorporation assay, by AA cell number counting, and by measuring AA cell protein content. Proliferation also occurs when AA cells are separated from the macrophage monolayer by millipore filters.
小鼠肉瘤MC1M的腹水亚系（AA）在体内可在腹腔中生长，但在玻璃或胶原蛋白上培养时在体外会死亡。AA细胞在体外与成纤维细胞系L929共培养时活力不受影响，而在添加巨噬细胞培养上清液的共培养物中或与非贴壁腹膜细胞共培养时活力会降低。然而，当与同基因、半同基因和异基因腹膜巨噬细胞共培养时，AA细胞可在玻璃或胶原蛋白上进行体外增殖。这通过氚标记胸腺嘧啶核苷掺入试验、AA细胞计数以及测量AA细胞蛋白含量得以证明。当AA细胞通过微孔滤膜与巨噬细胞单层分离时也会发生增殖。
