细胞视黄醇结合蛋白可使视黄醇在体外与细胞核发生特异性相互作用。
Cellular retinol-binding protein allows specific interaction of retinol with the nucleus in vitro.
作者信息
Takase S, Ong D E, Chytil F
出版信息
Proc Natl Acad Sci U S A. 1979 May;76(5):2204-8. doi: 10.1073/pnas.76.5.2204.
Abstract
Purified cellular retinol-binding protein (CRBP), a potential mediator of vitamin A action, was found to enable retinol to bind in a specific manner to isolated nuclei from livers of vitamin A deficient rats. Binding was followed after complexing [3H]retinol with CRBP. The binding was specific, saturable, and temperature dependent. CRBP charged with unlabeled retinol or CRBP without retinol diminished binding of radioactivity whereas free retinol did not. No specific binding sites could be detected for free retinol. Purified cellular retinoic acid binding protein (CRABI) complexed with retinoic acid did not diminish the amount of retinol bound to nuclei. Approximately 3 x 10(5) specific binding sites per nucleus could be detected. Fewer binding sites were found in nuclei isolated from livers of control (chow-fed) rats and also from livers of vitamin A-deficient rats 2 hr after refeeding with retinylacetate.
摘要
纯化的细胞视黄醇结合蛋白（CRBP）是维生素A作用的潜在介质，它能使视黄醇以特定方式与维生素A缺乏大鼠肝脏分离出的细胞核结合。将[3H]视黄醇与CRBP复合后跟踪结合情况。这种结合具有特异性、可饱和性且依赖温度。负载未标记视黄醇的CRBP或不含视黄醇的CRBP会减少放射性结合，而游离视黄醇则不会。未检测到游离视黄醇的特异性结合位点。与视黄酸复合的纯化细胞视黄酸结合蛋白（CRABI）不会减少与细胞核结合的视黄醇量。每个细胞核可检测到约3×10(5)个特异性结合位点。在从对照（正常饮食喂养）大鼠肝脏以及维生素A缺乏大鼠用视黄基乙酸酯重新喂养2小时后的肝脏中分离出的细胞核中发现的结合位点较少。
相似文献
1
Cellular retinol-binding protein allows specific interaction of retinol with the nucleus in vitro.细胞视黄醇结合蛋白可使视黄醇在体外与细胞核发生特异性相互作用。
Proc Natl Acad Sci U S A. 1979 May;76(5):2204-8. doi: 10.1073/pnas.76.5.2204.
2
Transfer of retinoic acid from its complex with cellular retinoic acid-binding protein to the nucleus.视黄酸从其与细胞视黄酸结合蛋白的复合物转移至细胞核。
Arch Biochem Biophys. 1986 Jun;247(2):328-34. doi: 10.1016/0003-9861(86)90591-6.
3
Interaction of the retinol/cellular retinol-binding protein complex with isolated nuclei and nuclear components.视黄醇/细胞视黄醇结合蛋白复合物与分离的细胞核及核成分的相互作用。
J Cell Biol. 1981 Oct;91(1):63-8. doi: 10.1083/jcb.91.1.63.
4
Analyses of the interactions between retinoid-binding proteins and embryonal carcinoma cells.视黄醇结合蛋白与胚胎癌细胞之间相互作用的分析。
J Cell Biol. 1987 Mar;104(3):671-8. doi: 10.1083/jcb.104.3.671.
5
Cellular retinol- and retinoic acid-binding proteins in vitamin A action.维生素A作用中的细胞视黄醇结合蛋白和视黄酸结合蛋白
Fed Proc. 1979 Oct;38(11):2510-4.
6
Specificity of cellular retinol-binding protein in the transfer of retinol to nuclei and chromatin.细胞视黄醇结合蛋白在视黄醇向细胞核和染色质转运中的特异性。
Arch Biochem Biophys. 1987 Apr;254(1):372-5. doi: 10.1016/0003-9861(87)90113-5.
7
Partial characterization of nuclear binding sites for retinol delivered by cellular retinol binding protein.细胞视黄醇结合蛋白所传递的视黄醇的核结合位点的部分特性分析
Arch Biochem Biophys. 1985 Mar;237(2):354-60. doi: 10.1016/0003-9861(85)90287-5.
8
Retinol bound to cellular retinol-binding protein is a substrate for cytosolic retinoic acid synthesis.与细胞视黄醇结合蛋白结合的视黄醇是胞质视黄酸合成的底物。
J Biol Chem. 1993 Dec 25;268(36):27133-42.
9
[Retinol transport into the cell nucleus in vitro].[视黄醇在体外向细胞核的转运]
Ukr Biokhim Zh (1978). 1998 Mar-Apr;70(2):15-21.
10
Cellular retinol-binding protein messenger RNA levels in normal and retinoid-deficient rats.正常和类维生素A缺乏大鼠体内细胞视黄醇结合蛋白信使核糖核酸水平
J Lipid Res. 1990 May;31(5):821-9.
引用本文的文献
1
Retinol-binding protein 2 (RBP2): biology and pathobiology.视黄醇结合蛋白 2（RBP2）：生物学与病理生物学。
Crit Rev Biochem Mol Biol. 2020 Apr;55(2):197-218. doi: 10.1080/10409238.2020.1768207. Epub 2020 May 28.
2
Tissue distribution of glycine N-methyltransferase, a major folate-binding protein of liver.
Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):210-4. doi: 10.1073/pnas.91.1.210.
3
Interaction of the retinol/cellular retinol-binding protein complex with isolated nuclei and nuclear components.视黄醇/细胞视黄醇结合蛋白复合物与分离的细胞核及核成分的相互作用。
J Cell Biol. 1981 Oct;91(1):63-8. doi: 10.1083/jcb.91.1.63.
4
Binding of retinoids to human breast cancer cell lines and their effects on cell growth.类视黄醇与人类乳腺癌细胞系的结合及其对细胞生长的影响。
J Clin Invest. 1980 Mar;65(3):586-91. doi: 10.1172/JCI109703.
5
Vitamin A (retinol) status in the Gunn rat. The effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin.冈恩大鼠体内的维生素A（视黄醇）状态。2,3,7,8-四氯二苯并对二恶英的影响。
Arch Toxicol. 1983 Jul;53(3):225-33. doi: 10.1007/BF00316506.
6
Localization of cellular retinol-binding protein in several rat tissues.细胞视黄醇结合蛋白在几种大鼠组织中的定位
Proc Natl Acad Sci U S A. 1983 Nov;80(21):6586-90. doi: 10.1073/pnas.80.21.6586.
7
Rat testes interstitial cell nuclei exhibit three distinct receptors for retinoic acid.大鼠睾丸间质细胞核表现出三种不同的视黄酸受体。
Experientia. 1984 Mar 15;40(3):276-7. doi: 10.1007/BF01947580.
8
Nuclear interactions of retinoic acid-binding protein in chemically induced mammary adenocarcinoma.化学诱导的乳腺腺癌中视黄酸结合蛋白的核相互作用
Biochem J. 1982 Dec 15;208(3):731-6. doi: 10.1042/bj2080731.
9
Induction of murine teratocarcinoma cell differentiation by suppression of poly(ADP-ribose) synthesis.通过抑制聚（ADP-核糖）合成诱导小鼠畸胎瘤细胞分化
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7132-6. doi: 10.1073/pnas.81.22.7132.
10
A chick neural retina adhesion and survival molecule is a retinol-binding protein.一种鸡神经视网膜黏附与存活分子是一种视黄醇结合蛋白。
J Cell Biol. 1986 Jun;102(6):2295-301. doi: 10.1083/jcb.102.6.2295.
本文引用的文献
1
Isolation of pure and unaltered liver nuclei morphology and biochemical composition.分离出形态和生化组成纯净且未改变的肝细胞核。
Exp Cell Res. 1956 Aug;11(2):317-21. doi: 10.1016/0014-4827(56)90107-0.
2
The stimulation of RNA synthesis in vivo and in vitro by retinol (vitamin A) in the intestine of vitamin A deficient rats.维生素A缺乏大鼠肠道中视黄醇（维生素A）对体内和体外RNA合成的刺激作用。
Life Sci. 1967 Oct 15;6(20):2207-13. doi: 10.1016/0024-3205(67)90244-5.
3
Studies on metabolism of vitamin A. 8. Effect of ascorbate on isoprenoid metabolism in vitamin A-deficient rats.维生素A代谢的研究。8. 抗坏血酸盐对维生素A缺乏大鼠类异戊二烯代谢的影响。
Biochem J. 1964 Sep;92(3):527-31. doi: 10.1042/bj0920527.
4
Vitamin A and nuclear RNA synthesis.维生素A与核RNA合成
Am J Clin Nutr. 1969 Aug;22(8):1048-58. doi: 10.1093/ajcn/22.8.1048.
5
Vitamin A and ribonucleic acid synthesis in rat intestine.维生素A与大鼠肠道中的核糖核酸合成
Arch Biochem Biophys. 1970 Sep;140(1):210-4. doi: 10.1016/0003-9861(70)90024-x.
6
Altered messenger RNA synthesis in vitamin A deficient rat liver.维生素A缺乏大鼠肝脏中信使核糖核酸合成的改变
Life Sci II. 1971 Oct 8;10(19):1097-103. doi: 10.1016/0024-3205(71)90261-x.
7
RNA metabolism in tracheal epithelium: alteration in hamsters deficient in vitamin A.
Science. 1972 Sep 22;177(4054):1105-8. doi: 10.1126/science.177.4054.1105.
8
Induction of rapid, synchronous vitamin A deficiency in the rat.在大鼠中诱导快速、同步的维生素A缺乏。
J Nutr. 1974 Sep;104(9):1140-8. doi: 10.1093/jn/104.9.1140.
9
Multiple retinol binding proteins in rabbit lung.兔肺中的多种视黄醇结合蛋白。
Biochem Biophys Res Commun. 1974 Jul 10;59(1):221-9. doi: 10.1016/s0006-291x(74)80196-8.
10
In vitro binding of retinol to rat-tissue components.视黄醇与大鼠组织成分的体外结合
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3483-7. doi: 10.1073/pnas.70.12.3483.
Zotero 插件