Van Charldorp R, Heus H A, Van Knippenberg P H
Nucleic Acids Res. 1981 Jan 24;9(2):267-75. doi: 10.1093/nar/9.2.267.
The electrophoretic mobility of RNA fragments derived from the 3'-end of 16S rRNA on slabs of polyacrylamide gel in the presence of urea is strongly influenced by dimethylation of the N6-aminogroup of two adjacent adenosines. This is not due to the presence of the methylgroups per se, but must be ascribed to an effect of methylation on long range intramolecular interactions at these denaturing conditions. When it is assumed that the electrophoretic mobilities of the RNA fragments in the polyacrylamide matrix are determined by the conformational state(s) of the fragments, dimethylation of the adenosines leads in the smaller fragments to a less compact average conformation and in the larger fragments to a more compact average conformation. An effort is made to comprehend the effects of adenosine dimethylation in terms of secondary structure based on nucleotide sequence.
在尿素存在的情况下，源自16S rRNA 3'端的RNA片段在聚丙烯酰胺凝胶平板上的电泳迁移率受到两个相邻腺苷N6-氨基二甲基化的强烈影响。这并非由于甲基本身的存在，而是必须归因于在这些变性条件下甲基化对长程分子内相互作用的影响。当假定聚丙烯酰胺基质中RNA片段的电泳迁移率由片段的构象状态决定时，腺苷的二甲基化导致较小片段的平均构象较不紧密，而较大片段的平均构象较紧密。人们努力根据核苷酸序列从二级结构的角度理解腺苷二甲基化的影响。
