Jeon Hongjun, Lim Chaemin, Lee Ji Min, Kim Sanghee
College of Pharmacy , Seoul National University , 1 Gwanak-ro, Gwanak-gu , Seoul 151-742 , Korea . Email:
Chem Sci. 2015 May 1;6(5):2806-2811. doi: 10.1039/c5sc00360a. Epub 2015 Mar 25.
As a new model of chemical assay-guided natural product isolation, an effective chemodosimetric assay system was devised. Our chemical assay system features a fluorogenic chemodosimeter immobilized on a solid support, which offers advantages in identifying the desired compounds in complex natural product mixtures. To isolate only compounds with the target functional groups, the click chemistry concept was adopted. The model system presented here was developed for natural products with a terminal alkyne. Using our newly designed alkyne sensing beads with the aforementioned features, we have readily identified, quantified, and isolated compounds with a terminal alkyne group from plant extracts.
