Department of Pharmaceutical Sciences, Dr. Hari Singh Gour University, Sagar 470003, MP, India.
Phytother Res. 2013 Jan;27(1):99-106. doi: 10.1002/ptr.4697. Epub 2012 Apr 4.
Anacyclus pyrethrum (A. pyrethrum) has been used as Vajikaran Rasayana (aphrodisiac) in traditional Indian ayurvedic medicine to treat male sexual dysfunction, including infertility. Aphrodisiac activity may be due to an increase in the production or effect of androgens, so this study sought to evaluate the androgenic and spermatogenic potential of the alkylamide-rich ethanol solution extract. Male Wistar strain rats weighing between 150 and 180 g were completely randomized divided into five groups. The ethanol solution extract of A. pyrethrum was administered to groups of rats in 50, 100, and 150 mg/kg doses for a period of 28 days, and the action was compared with control and testosterone-treated rats. Thirteen N-alkylamides were detected in the extract by using HPLC/UV/electrospray ionization mass spectrometry method. Extract administration at all the doses produced significant increase in body weight, sperm count, motility, and viability along with serum testosterone, luteinizing hormone, and follicle-stimulating hormone concentrations. Histoarchitecture of testis revealed increased spermatogenic activities. Seminal fructose content was also significantly increased after 28 days of treatment. Our results suggest that the ethanol solution extract of the roots of A. pyrethrum has androgenic potential and may improve male fertility by enhancing spermatogenesis.
春黄菊（A. pyrethrum）已被用作传统印度阿育吠陀医学中的 Vajikaran Rasayana（壮阳药），用于治疗男性性功能障碍，包括不育症。壮阳药的作用可能是由于雄激素的产生或作用增加，因此本研究旨在评估富含烷基酰胺的乙醇溶液提取物的雄激素和生精潜力。雄性 Wistar 品系大鼠体重在 150 至 180 克之间，完全随机分为五组。用 50、100 和 150mg/kg 剂量的 A. pyrethrum 乙醇溶液提取物给大鼠给药 28 天，并与对照组和睾酮处理的大鼠进行比较。使用 HPLC/UV/电喷雾电离质谱法在提取物中检测到 13 种 N-烷基酰胺。在所有剂量下，提取物的给药都会导致体重、精子数、活力和活力以及血清睾丸激素、促黄体生成素和卵泡刺激素浓度的显著增加。睾丸的组织学结构显示出增加的生精活性。治疗 28 天后，精子果糖含量也显著增加。我们的结果表明，春黄菊根的乙醇溶液提取物具有雄激素潜力，通过增强生精作用可能改善男性生育能力。
