Saha-Shah Anumita, Weber Anna E, Karty Jonathan A, Ray Steven J, Hieftje Gary M, Baker Lane A
Department of Chemistry , Indiana University , 800 E. Kirkwood Avenue , Bloomington , IN 47405 , USA . Email:
Chem Sci. 2015 Jun 1;6(6):3334-3341. doi: 10.1039/c5sc00668f. Epub 2015 Apr 13.
Nanopipettes (pipettes with diameters <1 μm) were explored as pressure-driven fluid manipulation tools for sampling nanoliter volumes of fluids. The fundamental behavior of fluids confined in the narrow channels of the nanopipette shank was studied to optimize sampling volume and probe geometry. This method was utilized to collect nanoliter volumes (<10 nL) of sample from single cells and live first instar larvae. Matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS) was utilized to characterize the collected sample. The use of nanopipettes for surface sampling of mouse brain tissue sections was also explored. Lipid analyses were performed on mouse brain tissues with spatial resolution of sampling as small as 50 μm. Nanopipettes were shown to be a versatile tool that will find further application in studies of sample heterogeneity and population analysis for a wide range of samples.
纳米移液器（直径<1μm的移液器）被用作压力驱动的流体操纵工具，用于采集纳升级体积的流体。研究了限制在纳米移液器柄部狭窄通道内的流体的基本行为，以优化采样体积和探针几何形状。该方法用于从单细胞和活的一龄幼虫中收集纳升级体积（<10 nL）的样品。利用基质辅助激光解吸/电离质谱（MALDI-MS）对收集的样品进行表征。还探索了使用纳米移液器对小鼠脑组织切片进行表面采样。对小鼠脑组织进行了脂质分析，采样空间分辨率小至50μm。纳米移液器被证明是一种多功能工具，将在广泛样品的样品异质性研究和群体分析中得到进一步应用。
