Bogdahn M, Kleiner D
Arch Microbiol. 1986 Feb;144(1):102-4. doi: 10.1007/BF00454964.
Inorganic nitrogen metabolism in the obligate anaerobic thermophiles Chlostridium thermosaccharolyticum and Clostridium thermoautotrophicum differs in several respects. C. thermosaccharolyticum contains a nitrogenase as inferred from NH4+ repressible C2H2 reduction, a glutamine synthetase which is partially repressed by ammonium, very labile glutamate synthase activities with both NADH and NADPH, NADPH-dependent glutamate dehydrogenase, and NH4+-dependent asparagine synthetase. C. thermoautotrophicum contains no nitrogenase, but glutamine synthetase, no glutamate synthase, no glutamate dehydrogenase, but a NADH-dependent alanine dehydrogenase and a NH4+-dependent asparagine synthetase.
专性厌氧嗜热菌解糖嗜热梭菌和自养嗜热梭菌的无机氮代谢在几个方面存在差异。从NH4+可抑制的C2H2还原可推断,解糖嗜热梭菌含有固氮酶、一种部分受铵抑制的谷氨酰胺合成酶、对NADH和NADPH都非常不稳定的谷氨酸合成酶活性、NADPH依赖性谷氨酸脱氢酶以及NH4+依赖性天冬酰胺合成酶。自养嗜热梭菌不含固氮酶,但含有谷氨酰胺合成酶,不含谷氨酸合成酶,不含谷氨酸脱氢酶,但含有NADH依赖性丙氨酸脱氢酶和NH4+依赖性天冬酰胺合成酶。