Giordani B F, Andrade A J, Galati E A B, Gurgel-Gonçalves R
Laboratório de Parasitologia Médica e Biologia de Vetores, Área de Patologia, Faculdade de Medicina, Universidade de Brasília, Brasilia, Brazil.
Laboratório de Parasitologia Molecular, Departamento de Patologia Básica, Setor de Ciências Biológicas, Universidade Federal do Paraná, Curitiba, Brazil.
Med Vet Entomol. 2017 Dec;31(4):373-380. doi: 10.1111/mve.12245. Epub 2017 Jul 14.
The Lutzomyia subgenus (Diptera: Psychodidae) includes sibling species with morphologically indistinguishable females. The aims of this study were to analyse variations in the size and shape of wings of species within the Lutzomyia subgenus and to assess whether these analyses might be useful in their identification. Wings (n = 733) of 18 species deposited in Brazilian collections were analysed by geometric morphometrics, using other genera and subgenera as outgroups. Shape variation was summarized in multivariate analyses and differences in wing size among species were tested by analysis of variance. The results showed significant variation in the sizes and shapes of wings of different Lutzomyia species. Two clusters within the Lutzomyia subgenus were distinguished in analyses of both males and females. In Cluster 1 (Lutzomyia ischnacantha, Lutzomyia cavernicola, Lutzomyia almerioi, Lutzomyia forattinii, Lutzomyia renei and Lutzomyia battistinii), scores for correct reclassification were high (females, kappa = 0.91; males, kappa = 0.90), whereas in Cluster 2 (Lutzomyia alencari, Lutzomyia ischyracantha, Lutzomyia cruzi, Lutzomyia longipalpis, Lutzomyia gaminarai and Lutzomyia lichyi), scores for correct reclassification were low (females, kappa = 0.42; males, kappa = 0.48). Wing geometry was useful in the identification of some species of the Lutzomyia subgenus, but did not allow the identification of sibling species such as L. longipalpis and L. cruzi.
白蛉亚属（双翅目：毛蠓科）包括雌性在形态上难以区分的姐妹种。本研究的目的是分析白蛉亚属内各物种翅膀大小和形状的变化，并评估这些分析是否有助于它们的鉴定。通过几何形态测量学分析了保存在巴西标本馆中的18个物种的733只翅膀（以其他属和亚属作为外类群）。在多变量分析中总结了形状变化，并通过方差分析检验了物种间翅膀大小的差异。结果表明，不同白蛉物种的翅膀大小和形状存在显著差异。在对白蛉亚属雄性和雌性的分析中都区分出了两个类群。在类群1（伊斯纳卡纳白蛉、洞穴白蛉、阿尔梅里奥伊白蛉、福拉蒂尼白蛉、雷内白蛉和巴蒂斯蒂尼白蛉）中，正确重新分类的得分较高（雌性，卡帕系数=0.91；雄性，卡帕系数=0.90），而在类群2（阿伦卡里白蛉、伊斯奇拉坎塔白蛉、克氏白蛉、长须白蛉、加米纳拉伊白蛉和利奇伊白蛉）中，正确重新分类的得分较低（雌性，卡帕系数=0.42；雄性，卡帕系数=0.48）。翅膀几何形态测量有助于白蛉亚属某些物种的鉴定，但无法区分长须白蛉和克氏白蛉等姐妹种。
