Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, People's Republic of China.
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Shanghai, 200050, People's Republic of China.
J Biomed Mater Res A. 2017 Nov;105(11):3093-3101. doi: 10.1002/jbm.a.36157. Epub 2017 Aug 22.
Micropatterning technique has been used to control single cell geometry in many researches, however, this is no report that it is used to control multicelluar geometry, which not only control single cell geometry but also organize those cells by a certain pattern. In this work, a composite protein micropattern is developed to control both cell shape and cell location simultaneously. The composite micropattern consists of a central circle 15 μm in diameter for single-cell capture, surrounded by small, square arrays (3 μm × 3 μm) for cell spreading. This is surrounded by a border 2 μm wide for restricting cell edges. The composite pattern results in two-cell and three-cell capture efficiencies of 32.1% ± 1.94% and 24.2% ± 2.89%, respectively, representing an 8.52% and 9.58% increase, respectively, over rates of original patterns. Fluorescent imaging of cytoskeleton alignment demonstrates that actin is gradually aligned parallel to the direction of the entire pattern arrangement, rather than to that of a single pattern. This indicates that cell arrangement is also an important factor in determining cell physiology. This composite micropattern could be a potential method to precisely control multi-cells for cell junctions, cell interactions, cell signal transduction, and eventually for tissue rebuilding study. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 3093-3101, 2017.
微图案化技术已被用于控制许多研究中的单细胞几何形状，但是，尚无报告表明它可用于控制多细胞的几何形状，因为它不仅可以控制单细胞的几何形状，还可以通过特定的图案对细胞进行组织排列。在这项工作中，开发了一种复合蛋白微图案来同时控制细胞形状和位置。复合微图案由一个直径为 15μm 的中央圆组成，用于捕获单细胞，周围是用于细胞扩展的小正方形阵列（3μm×3μm）。这被一个 2μm 宽的边框包围，用于限制细胞边缘。复合图案的两细胞和三细胞捕获效率分别为 32.1%±1.94%和 24.2%±2.89%，分别比原始图案的捕获效率提高了 8.52%和 9.58%。细胞骨架排列的荧光成像表明，肌动蛋白逐渐沿整个图案排列的方向平行排列，而不是沿单个图案的方向排列。这表明细胞排列也是决定细胞生理学的重要因素。这种复合微图案可能是一种精确控制多细胞用于细胞连接、细胞相互作用、细胞信号转导，最终用于组织重建研究的潜在方法。©2017 年 Wiley 期刊，Inc. J Biomed Mater Res Part A：105A：3093-3101，2017。
