Zou Xingxing, Lin Jinping, Mao Xinlei, Zhao Shengyun, Ren Yuhong
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology , Shanghai 200237, China.
Fujian Key Laboratory of Eco-Industrial Green Technology, Wuyi University , Wuyishan, 354300, China.
J Agric Food Chem. 2017 Sep 6;65(35):7721-7725. doi: 10.1021/acs.jafc.7b02201. Epub 2017 Aug 24.
L-erythrose, a rare aldotetrose, possesses various pharmacological activities. However, efficient L-erythrose production is challenging. Currently, L-erythrose is produced by a two-step fermentation process from erythritol. Here, we describe a novel strategy for the production of L-erythrose in Gluconobacter oxydans (G. oxydans) by localizing the assembly of L-ribose isomerase (L-RI) to membrane-bound sorbitol dehydrogenase (SDH) via the protein-peptide interactions of the PDZ domain and PDZ ligand. To demonstrate this self-assembly, green fluorescent protein (GFP) replaced L-RI and its movement to membrane-bound SDH was observed by fluorescence microscopy. The final L-erythrose production was improved to 23.5 g/L with the stepwise metabolic engineering of G. oxydans, which was 1.4-fold higher than that obtained using coexpression of SDH and L-RI in G. oxydans. This self-assembly strategy shows remarkable potential for further improvement of L-erythrose production.
L-赤藓糖是一种罕见的醛糖四糖，具有多种药理活性。然而，高效生产L-赤藓糖具有挑战性。目前，L-赤藓糖是通过从赤藓糖醇进行两步发酵过程生产的。在此，我们描述了一种在氧化葡萄糖杆菌（G. oxydans）中生产L-赤藓糖的新策略，即通过PDZ结构域和PDZ配体的蛋白质-肽相互作用将L-核糖异构酶（L-RI）的组装定位到膜结合的山梨醇脱氢酶（SDH）上。为了证明这种自组装，用绿色荧光蛋白（GFP）取代L-RI，并通过荧光显微镜观察其向膜结合SDH的移动。通过对氧化葡萄糖杆菌进行逐步代谢工程，最终L-赤藓糖产量提高到23.5 g/L，比在氧化葡萄糖杆菌中共表达SDH和L-RI所获得的产量高1.4倍。这种自组装策略显示出进一步提高L-赤藓糖产量的巨大潜力。
