Jaafar R M, Al-Jubury A, Chettri J K, Dalsgaard I, Kania P W, Buchmann K
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg C, Denmark.
National Veterinary Institute, Technical University of Denmark, Frederiksberg C, Denmark.
J Fish Dis. 2018 Jan;41(1):117-123. doi: 10.1111/jfd.12682. Epub 2017 Jul 14.
Teleosts are able to raise a protective immune response, comprising both innate and adaptive elements, against various pathogens. This is the basis for a widespread use of vaccines, administered as injection or immersion, in the aquaculture industry. It has been described that repeated injection vaccination of fish raises a secondary immune response, consisting of rapid, accelerated and increased antibody reaction. This study reports how rainbow trout responds to repeated immersion vaccination against yersiniosis (ERM) caused by the bacterial pathogen Yersinia ruckeri. It was found that rainbow trout does not raise a classical secondary response following repeated immersion vaccination. Serum antibody titres were merely slightly increased even after three immunizations, using 30-s immersion into a bacterin consisting of formalin-inactivated Y. ruckeri (serotype O1, biotypes 1 and 2), performed over a 3-month period. The densities of IgM-positive lymphocytes in spleen of fish immunized three times were increased compared to control fish, but no general trend for an increase with the number of immunizations was noted. The lack of a classical secondary response following repeated immersion vaccination may partly be explained by limited uptake of antigen by immersion compared to injection.
硬骨鱼能够针对各种病原体产生包括先天和适应性成分的保护性免疫反应。这是水产养殖业广泛使用通过注射或浸泡方式接种的疫苗的基础。据描述，对鱼类进行重复注射疫苗接种会引发二次免疫反应，包括快速、加速和增强的抗体反应。本研究报告了虹鳟鱼对针对由细菌病原体鲁氏耶尔森菌引起的耶尔森菌病（ERM）的重复浸泡疫苗接种的反应。研究发现，虹鳟鱼在重复浸泡疫苗接种后不会产生典型的二次反应。即使在3个月内进行了三次免疫接种，每次30秒浸泡于由福尔马林灭活的鲁氏耶尔森菌（血清型O1，生物型1和2）组成的菌苗中，血清抗体滴度也仅略有增加。与对照鱼相比，三次免疫接种的鱼脾脏中IgM阳性淋巴细胞的密度增加，但未观察到随着免疫接种次数增加而增加的总体趋势。重复浸泡疫苗接种后缺乏典型的二次反应，部分原因可能是与注射相比，通过浸泡摄取的抗原有限。
