Preventive Veterinary Microbiology, Department of Veterinary and Animal Sciences, University of Copenhagen, Frederiksberg, Denmark.
Division of Development of Functional Brain Activities,Research Centre for Child Mental Development, University of Fukui, Fukui, Japan.
Sci Rep. 2020 Oct 12;10(1):16993. doi: 10.1038/s41598-020-73812-8.
Enteric redmouth disease caused by the bacterial pathogen Yersinia ruckeri is the main reason for antimicrobial prescription, and a cause of substantial economic losses and decreased animal welfare in aquaculture. Given the importance of the intestinal microbiota in digestion and disease, our aim was to investigate whether synbiotic feed supplementation strategies could improve feed performance and disease resistance. Four experimental synbiotic feeds formulated with pre- and probiotics were tested against a commercially available probiotic control feed. Each experimental feed was evaluated for feed performance, effects on gross as well as intestinal morphometrics, and finally their effect on resistance against a waterborne experimental infection with Yersinia ruckeri serotype O1, biotype 2. While co-supplementing Pediococcus acidilactici with citrus flavonoids or bacterial paraprobiotics significantly improved utilization of feed lipid content relative to the control group, a decrease in lipid utilization was observed for feeds that combined P. acidilactici with yeast paraprobiotics. No significant improvements on disease resistance were observed. Still, synbiotic formulations including P. acidilactici led to reduced risks relative to that of the control group, while an increased relative risk was observed for a Bacillus-based formulation. In conclusion, two of the synbiotic supplements significantly improved lipid utilization and contributed to minor increases in disease resistance.
由细菌病原体鲁氏耶尔森菌引起的肠型红嘴病是使用抗菌药物的主要原因，也是水产养殖中造成重大经济损失和动物福利下降的原因之一。鉴于肠道微生物群在消化和疾病方面的重要性，我们的目的是研究共生饲料补充策略是否可以提高饲料性能和疾病抵抗力。我们用益生菌和益生元测试了四种实验性共生饲料，与市售的益生菌对照饲料进行了对比。评估了每种实验性饲料的饲料性能、对总体和肠道形态计量学的影响，最后评估了它们对水生性鲁氏耶尔森菌 O1 血清型 2 型实验性感染的抵抗力。虽然嗜酸乳杆菌与柑橘类黄酮或细菌副生素的共同补充相对于对照组显著提高了饲料脂质含量的利用率，但将嗜酸乳杆菌与酵母副生素结合使用时，发现脂质利用率降低。对疾病抵抗力没有明显的改善。尽管如此，包含嗜酸乳杆菌的共生配方的风险相对于对照组降低，而基于芽孢杆菌的配方的相对风险增加。总之，两种共生补充剂显著提高了脂质利用率，并对疾病抵抗力的提高略有贡献。
