钠诱导棕色脂肪组织中线粒体释放钙。
Sodium-induced calcium release from mitochondria in brown adipose tissue.
作者信息
Al-Shaikhaly M H, Nedergaard J, Cannon B
出版信息
Proc Natl Acad Sci U S A. 1979 May;76(5):2350-3. doi: 10.1073/pnas.76.5.2350.
Abstract
Coupled mitochondria of brown adipose tissue can accumulate Ca2+ if a substrate is present. The Ca2+ is released by addition of 20 mM Na+, but not by addition of K+ or choline +. Energy-dissipating Na+-induced Ca2+ cycling occurs maximally with 20 mM Na+ and 10 microM Ca2+. In brown adipocytes, the Ca2+ ionophore A23187 and the Na+ ionophore monensin increase respiration if substrate is added, and incubation in a low-Na+ buffer decreases norepinephrine-induced respiration. Thus Na+-induced Ca2+ release takes place in brown adipose tissue; released Ca2+ could have a regulatory or thermogenic role or both.
摘要
如果存在底物，棕色脂肪组织的偶联线粒体可以积累Ca2+。加入20 mM Na+可释放Ca2+，但加入K+或胆碱+则不能。在20 mM Na+和10 microM Ca2+时，能量耗散的Na+诱导的Ca2+循环达到最大值。在棕色脂肪细胞中，如果加入底物，Ca2+离子载体A23187和Na+离子载体莫能菌素会增加呼吸作用，而在低Na+缓冲液中孵育会降低去甲肾上腺素诱导的呼吸作用。因此，Na+诱导的Ca2+释放在棕色脂肪组织中发生；释放的Ca2+可能具有调节作用或产热作用，或两者兼有。
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本文引用的文献
1
Determination of serum proteins by means of the biuret reaction.通过双缩脲反应测定血清蛋白。
J Biol Chem. 1949 Feb;177(2):751-66.
2
Membrane potential of brown adipose tissue. A suggested mechanism for the regulation of thermogenesis.棕色脂肪组织的膜电位。一种调节产热的推测机制。
J Gen Physiol. 1968 Dec;52(6):925-40. doi: 10.1085/jgp.52.6.925.
3
Calcium uptake and its effect on respiration and phosphorylation in mitochondria from brown adipose tissue.钙摄取及其对棕色脂肪组织线粒体呼吸和磷酸化的影响。
Eur J Biochem. 1971 Mar 11;19(2):276-82. doi: 10.1111/j.1432-1033.1971.tb01315.x.
4
Oxidative phosphorylation and compartmentation of fatty acid metabolism in brown fat mitochondria.棕色脂肪线粒体中脂肪酸代谢的氧化磷酸化与区室化
Eur J Biochem. 1969 Nov;11(1):183-92. doi: 10.1111/j.1432-1033.1969.tb00759.x.
5
Effect in vivo of norepinephrine on the membrane resistance of brown fat cells.去甲肾上腺素对棕色脂肪细胞膜电阻的体内效应。
Experientia. 1971 Dec 15;27(12):1419-21. doi: 10.1007/BF02154265.
6
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J Mol Cell Cardiol. 1974 Aug;6(4):361-71. doi: 10.1016/0022-2828(74)90077-7.
7
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Am J Physiol. 1973 Jun;224(6):1300-4. doi: 10.1152/ajplegacy.1973.224.6.1300.
8
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Am J Physiol. 1973 Feb;224(2):352-5. doi: 10.1152/ajplegacy.1973.224.2.352.
9
The effect of ruthenium red on Ca 2+ transport and respiration in rat liver mitochondria.
Biochim Biophys Acta. 1972 Jan 21;256(1):43-54. doi: 10.1016/0005-2728(72)90161-2.
10
Norepinephrine-sensitive Na+-K+ ATPase activity in brown adipose tissue.棕色脂肪组织中对去甲肾上腺素敏感的钠钾ATP酶活性
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