Rakha B A, Ansari M S, Akhter S, Blesbois E
Department of Wildlife Management, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan.
Department of Zoology, University of Sargodha, Lyallpur Campus, Faisalabad 38000, Pakistan.
Theriogenology. 2017 Sep 1;99:105-110. doi: 10.1016/j.theriogenology.2017.05.016. Epub 2017 May 24.
The reproductive potential of the adult males is expected to vary with age/season and largely differ not only in closely related avian species but even in subspecies, breeds and/or strains of the same species. Thus, it is pre-requisite to have knowledge of seminal parameters to achieve maximum production potential of at-risk species for ex situ in vitro conservation programs. A 4-year study was designed to evaluate the effect of age and season (spring, summer, autumn and winter) on semen characteristics of Indian red jungle fowl (Gallus gallus murghi) in a retrospective manner. Semen ejaculates (n = 1148) were regularly collected from eight adult cocks 6 to 54 months of age. Quantitative and qualitative semen parameters viz; volume (μL), concentration (1 × 10 mL), total sperm number per ejaculate (1 × 10 mL), motility (%), viability (%), plasma membrane integrity (%), acrosome integrity (%) and semen quality factor were recorded. A chronological increasing trend with age of most sperm quantitative and qualitative traits (semen volume, sperm concentration, total sperm number per ejaculate, plasma membrane integrity, viability, acrosomal integrity and semen quality factor) was observed. The highest values were observed at four years of age (P < 0.05) with the exception of sperm motility that was not affected by the age. Spring was the best season for sperm parameters viz; volume, motility, plasma membrane integrity, viability and acrosomal integrity (P < 0.05), however a remarkable sperm production was noticed all over the year. It is concluded that Indian red jungle fowl exhibits an evolution of sperm production that greatly differs in many points from other fowl sub-species. It is suggested that semen ejaculates of highest quality achieved for semen banking at the age of four year in the spring season.
成年雄性的繁殖潜力预计会随年龄/季节而变化，不仅在亲缘关系密切的鸟类物种中差异很大，甚至在同一物种的亚种、品种和/或品系中也有很大不同。因此，了解精液参数是实现濒危物种异地体外保存计划最大生产潜力的先决条件。一项为期4年的研究旨在以回顾性方式评估年龄和季节（春季、夏季、秋季和冬季）对印度红原鸡（Gallus gallus murghi）精液特征的影响。从8只6至54月龄的成年公鸡定期采集精液射精样本（n = 1148）。记录精液的定量和定性参数，即体积（μL）、浓度（1×10/mL）、每次射精的总精子数（1×10/mL）、活力（%）、存活率（%）、质膜完整性（%）、顶体完整性（%）和精液质量因子。观察到大多数精子定量和定性特征（精液体积、精子浓度、每次射精的总精子数、质膜完整性、存活率、顶体完整性和精液质量因子）随年龄呈时间顺序增加趋势。除精子活力不受年龄影响外，4岁时观察到最高值（P < 0.05）。春季是精液参数（即体积、活力、质膜完整性、存活率和顶体完整性）的最佳季节（P < 0.05），不过全年都有显著的精子产生。结论是，印度红原鸡表现出精子产生的进化，在许多方面与其他家禽亚种有很大不同。建议在春季4岁时采集用于精液库保存的最高质量精液射精样本。
