使用基于菠萝蜜种子粉的培养基生产黄原胶：优化与表征
Xanthan gum production using jackfruit-seed-powder-based medium: optimization and characterization.
作者信息
Felicia Katherine R, Muthukumaran C, Sharmila G, Manoj Kumar N, Tamilarasan K, Jaiganesh R
机构信息
Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur, Chennai, 603 203, India.
Department of Industrial Biotechnology, Government College of Technology, Coimbatore, 641 013, India.
出版信息
3 Biotech. 2017 Aug;7(4):248. doi: 10.1007/s13205-017-0876-5. Epub 2017 Jul 15.
Xanthan gum (XG) production by Xanthomonas campestris NCIM 2961 using jackfruit seed powder (JSP) as a novel substrate was reported. Central composite design (CCD) of response surface method (RSM) was used to evaluate the linear and interaction effects of five medium variables (JSP, peptone, citric acid, KHPO and KHPO) for XG production. Maximum XG production (51.62 g/L) was observed at the optimum level of JSP (4 g/L), peptone (0.93 g/L), citric acid (0.26 g/L), KHPO (1.29 g/L) and KHPO (0.5 g/L). KHPO and KHPO were found as significant medium components, which served as buffering agents as well as nutrients for X. campestris growth. The obtained biopolymer was characterized as XG by XRD and FTIR analysis. Results of this study revealed that JSP was found to be a suitable low cost substrate for XG production.
据报道，野油菜黄单胞菌NCIM 2961利用菠萝蜜种子粉（JSP）作为新型底物生产黄原胶（XG）。采用响应面法（RSM）的中心复合设计（CCD）来评估五个培养基变量（JSP、蛋白胨、柠檬酸、KHPO和KHPO）对XG生产的线性和交互作用。在JSP（4 g/L）、蛋白胨（0.93 g/L）、柠檬酸（0.26 g/L）、KHPO（1.29 g/L）和KHPO（0.5 g/L）的最佳水平下，观察到最大XG产量（51.62 g/L）。发现KHPO和KHPO是重要的培养基成分，它们既作为缓冲剂，又作为野油菜黄单胞菌生长的营养物质。通过XRD和FTIR分析将获得的生物聚合物表征为XG。本研究结果表明，JSP是一种适合用于XG生产的低成本底物。
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