Ozdal Murat, Kurbanoglu Esabi Basaran
Department of Biology, Faculty of Science, Ataturk University, Erzurum, Turkey.
J Genet Eng Biotechnol. 2018 Dec;16(2):259-263. doi: 10.1016/j.jgeb.2018.07.005. Epub 2018 Jul 24.
Xanthan gum is an important commercial polysaccharide produced by species. In this study, xanthan production was investigated using a local isolate of MO-03 in medium containing various concentrations of chicken feather peptone (CFP) as an enhancer substrate. CFP was produced with a chemical process and its chemical composition was determined. The addition of CFP (1-8 g/l) increased the conversion of sugar to xanthan gum in comparison with the control medium, which did not contain additional supplements. The highest xanthan production (24.45 g/l) was found at the 6 g/l CFP containing control medium in 54 h. This value was 1.73 fold higher than that of control medium (14.12 g/l). Moreover, addition of CFP improved the composition of xanthan gum; the pyruvate content of xanthan was 3.86% (w/w), higher than that of the control (2.2%, w/w). The xanthan gum yield was also influenced by the type of organic nitrogen sources. As a conclusion, CFP was found to be a suitable substrate for xanthan gum production.
黄原胶是由某些菌种产生的一种重要的商业多糖。在本研究中,使用MO - 03的本地分离株在含有不同浓度鸡毛蛋白胨(CFP)作为增强底物的培养基中研究黄原胶的生产。CFP通过化学方法制备并测定了其化学组成。与不含额外补充剂的对照培养基相比,添加CFP(1 - 8 g/l)提高了糖向黄原胶的转化率。在含6 g/l CFP的对照培养基中,54小时时黄原胶产量最高(24.45 g/l)。该值比对照培养基(14.12 g/l)高1.73倍。此外,添加CFP改善了黄原胶的组成;黄原胶的丙酮酸含量为3.86%(w/w),高于对照(2.2%,w/w)。黄原胶产量也受有机氮源类型的影响。总之,发现CFP是黄原胶生产的合适底物。