Tahir D, Davoust B, Almeras L, Berenger J M, Varloud M, Parola P
Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes (URMITE), Aix-Marseille Université, UM63, Centre National de la Recherche Scientifique (CNRS) 7278, Institut de Recherche pour le Développement (IRD) 198 (Dakar), Institut National de la Santé et de la Recherche Médicale (INSERM) 1095, Assistance-Publique Hôpitaux de Marseille (AP-HM) Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
Unité de Parasitologie et Entomologie, Département des Maladies Infectieuses, Institut de Recherche Biomédicale des Armées, Marseille, France.
Med Vet Entomol. 2017 Dec;31(4):351-357. doi: 10.1111/mve.12243. Epub 2017 Jul 17.
An ectoparasiticide combining three active ingredients [dinotefuran, permethrin and pyriproxyfen (DPP)] was used in mice in an experiment designed to evaluate its anti-feeding and insecticidal efficacy against Stegomyia albopicta (= Aedes albopictus) (Diptera: Culicidae) mosquitoes. Twenty-two adult mice were randomly allocated into two groups consisting of an untreated control group and a DPP-treated group. Mice were exposed individually for 1 h to a mean ± standard deviation of 27 ± 2 starved female mosquitoes on days 1, 7, 14, 21 and 28 post-treatment. At the end of the exposure (1 h), mosquitoes were assessed for immediate survival and engorgement status. Additionally, live mosquitoes in both groups were incubated separately and observed for mortality at 24 h after the end of the exposure. The anti-feeding efficacy of DPP after the 1-h exposure period was 99.2, 100, 98.0, 89.3 and 87.4% at 1, 7, 14, 21 and 28 days, respectively. Levels of insecticidal efficacy evaluated at 1 h and 24 h after exposure on days 1, 7, 14, 21 and 28 were 36.7, 28.9, 30.8, 23.1 and 11.9%, and 68.4, 45.0, 43.3, 37.9 and 19.9%, respectively. Based on the mouse model, the present study demonstrates that the DPP combination has significant anti-feeding and insecticidal efficacy against S. albopicta for at least 4 weeks.
一种含有三种活性成分[呋虫胺、氯菊酯和吡丙醚（DPP）]的体外寄生虫杀虫剂，被用于小鼠实验，该实验旨在评估其对白纹伊蚊（=埃及伊蚊）（双翅目：蚊科）蚊子的拒食和杀虫效果。22只成年小鼠被随机分为两组，一组为未处理的对照组，另一组为DPP处理组。在处理后的第1、7、14、21和28天，将小鼠分别暴露于平均±标准差为27±2只饥饿雌蚊1小时。暴露结束时（1小时），评估蚊子的即时存活和饱食状态。此外，两组中的活蚊子分别进行孵化，并在暴露结束后24小时观察死亡率。在暴露1小时后，DPP在第1、7、14、21和28天的拒食效果分别为99.2%、100%、98.0%、89.3%和87.4%。在第1、7、14、21和28天暴露后1小时和24小时评估的杀虫效果水平分别为36.7%、28.9%、30.8%、23.1%和11.9%，以及68.4%、45.0%、43.3%、37.9%和19.9%。基于小鼠模型，本研究表明DPP组合对白纹伊蚊具有显著的拒食和杀虫效果，且至少持续4周。
