Gurusmatika Sellen, Nishi Kosuke, Harmayani Eni, Pranoto Yudi, Sugahara Takuya
Department of Bioscience, Graduate School of Agriculture, Ehime University, Matsuyama, Ehime 790-8566, Japan.
Faculty of Agricultural Technology, Gadjah Mada University, Bulaksumur, Yogyakarta 55281, Indonesia.
Molecules. 2017 Jul 15;22(7):1187. doi: 10.3390/molecules22071187.
Porang is a local plant of Indonesia, which has a high content of glucomannan. In this study, porang glucomannan (PG) was esterified with octenyl succinic anhydride (OSA) to enhance emulsion properties to be widely used in food industry. OSA-modified PG (OSA-PG) enhanced the phagocytosis activity of macrophage-like J774.1 cells and mouse peritoneal macrophages. In addition, OSA-PG increased the production of IL-6 and TNF-α by enhancing their gene expression. Immunoblot analysis displayed that OSA-PG tended to activate both nuclear factor-κB and mitogen-activated protein kinase cascades. Treatment of OSA-PG with polymyxin B revealed that cytokine production induced by OSA-PG was not caused by endotoxin contamination. Our findings also indicated that OSA-PG activates macrophages through not only Toll-like receptor (TLR) 4, but another receptor. Overall findings suggested that OSA-PG has a potential as an immunomodulatory food factor by stimulating macrophages.
竹芋是印度尼西亚的本土植物，其葡甘露聚糖含量很高。在本研究中，竹芋葡甘露聚糖（PG）与辛烯基琥珀酸酐（OSA）进行酯化反应以增强乳化性能，从而在食品工业中得到广泛应用。OSA修饰的PG（OSA-PG）增强了巨噬细胞样J774.1细胞和小鼠腹腔巨噬细胞的吞噬活性。此外，OSA-PG通过增强IL-6和TNF-α的基因表达来增加它们的产生。免疫印迹分析表明，OSA-PG倾向于激活核因子-κB和丝裂原活化蛋白激酶级联反应。用多粘菌素B处理OSA-PG表明，OSA-PG诱导的细胞因子产生不是由内毒素污染引起的。我们的研究结果还表明，OSA-PG不仅通过Toll样受体（TLR）4激活巨噬细胞，还通过另一种受体激活。总体研究结果表明，OSA-PG通过刺激巨噬细胞具有作为免疫调节食品因子的潜力。
