Kytaro, Inc., Miami, FL, 33199, USA.
Department of Electrical and Computer Engineering, Florida International University, Miami, 33199, Florida, USA.
Sci Rep. 2017 Jul 17;7(1):5552. doi: 10.1038/s41598-017-05854-4.
A simple technique that employs an antibody coated polydimethylsiloxane tube is used for effective capturing of bloodborne and foodborne pathogens. By recirculating the entire sample through the antibody coated tube, accumulation of target pathogens is achieved, thereby delivering a higher concentration of pathogens in a small volume. The described method can provide an effective and economical solution to microbiology techniques that rely on enrichment, thereby expediting diagnostics. Using this method 80.3 ± 5.6% of Staphylococcus aureus with a starting concentration of ~10 CFU/mL and 95.4 ± 1.0% of Methicillin-resistant Staphylococcus aureus with starting concentration of ~10 CFU/mL were removed from 5 mL blood in a few hours. This concept was extended to live rats with an induced bloodstream S. aureus infection. A reduction of two orders of magnitude in the bacterial load of the rats was observed within a few hours. The same technique was used to capture a food pathogen, Salmonella typhimurium, with starting concentrations as low as ~10 CFU, from 100 or 250 mL of culture broth within similar timeframes as above. The feasibility for food pathogen testing applications was additionally confirmed by capturing and detecting S. typhimurium in ground chicken and ground beef.
一种简单的技术，使用涂有抗体的聚二甲基硅氧烷管，可有效捕获血源和食源性病原体。通过使整个样品在抗体涂覆的管中再循环，实现了目标病原体的积累，从而在小体积中提供了更高浓度的病原体。所描述的方法可以为依赖于富集的微生物学技术提供有效且经济的解决方案，从而加快诊断速度。使用该方法，从 5 毫升血液中在数小时内去除了起始浓度约为 10 CFU/mL 的 80.3±5.6%金黄色葡萄球菌和起始浓度约为 10 CFU/mL 的 95.4±1.0%耐甲氧西林金黄色葡萄球菌。该概念扩展到了诱导血流金黄色葡萄球菌感染的活体大鼠。在数小时内，观察到大鼠细菌负荷减少了两个数量级。相同的技术用于从 100 或 250 毫升培养肉汤中捕获起始浓度低至约 10 CFU 的食源性病原体鼠伤寒沙门氏菌。通过在类似的时间范围内从鸡肉和碎牛肉中捕获和检测鼠伤寒沙门氏菌，进一步证实了该技术用于食品病原体检测应用的可行性。
