Zhang Hongyan, Jia Zhenzhen, Wu Chuanchen, Zang Liguo, Yang Guiwen, Chen Zhenzhen, Tang Bo
College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Normal University , Jinan, Shandong 250014, P. R. China.
College of Life Science, Shandong Normal University , Jinan, Shandong 250014, P. R. China.
ACS Appl Mater Interfaces. 2015 Sep 16;7(36):20477-84. doi: 10.1021/acsami.5b06874. Epub 2015 Sep 4.
Detection of circulating tumor cells (CTCs) could be used as a "liquid biopsy" for tracking the spread of cancer. In vitro detection methods based on blood sampling and in vitro CTC capture often suffer from the small sampling volume and sampling error. Here, the in vivo capture of CTCs based on transfusion with a surface-modified vein indwelling needle is proposed. When the needle was applied to transfusion in the vein, the simultaneous capture of CTCs was performed. To investigate the actual capture efficiency of the in vivo capture method, labeled MCF-7 cells were directly injected into the veins of rabbits, wild type mice, and nude mice and could be successfully captured. Two of 5 MCF-7 cells injected into the veins of nude mice were successfully captured. To investigate the CTC capture of mouse tumor model and compare with the in vitro method, mice were subcutaneous inoculated with metastatic 4T1 cells. Seven and 21 days after inoculation, CTCs were captured for the first time using in vivo and in vitro methods, respectively. This predicted that the in vivo method could be more suitable for use of early diagnosis of cancer than the in vitro method. As CTC capture can be performed at the same time as transfusion and does not cause further bodily harm, it would be easily accepted by patients. This efficient, simple, and less damaging method involving the use of a vein indwelling needle could be popularized easily in the clinic.
循环肿瘤细胞（CTC）的检测可作为一种“液体活检”手段用于追踪癌症的扩散。基于血液采样和体外CTC捕获的体外检测方法常常存在采样量小和采样误差的问题。在此，我们提出了一种基于使用表面修饰的静脉留置针进行输血来在体内捕获CTC的方法。当该针应用于静脉输血时，可同时进行CTC的捕获。为了研究这种体内捕获方法的实际捕获效率，将标记的MCF-7细胞直接注入兔子、野生型小鼠和裸鼠的静脉中，并且这些细胞能够被成功捕获。注入裸鼠静脉的5个MCF-7细胞中有2个被成功捕获。为了研究小鼠肿瘤模型的CTC捕获情况并与体外方法进行比较，将转移性4T1细胞皮下接种到小鼠体内。接种后7天和21天，分别首次使用体内和体外方法捕获CTC。这预示着与体外方法相比，体内方法可能更适合用于癌症的早期诊断。由于CTC捕获可在输血的同时进行且不会对身体造成进一步伤害，它很容易被患者接受。这种涉及使用静脉留置针的高效、简单且损伤较小的方法可在临床上轻松推广。
