Nguyen Huong Mai, Sako Kaori, Matsui Akihiro, Suzuki Yuya, Mostofa Mohammad Golam, Ha Chien Van, Tanaka Maho, Tran Lam-Son Phan, Habu Yoshiki, Seki Motoaki
Plant Genomic Network Research Team, RIKEN Center for Sustainable Resource Science (CSRS)Yokohama, Japan.
Kihara Institute for Biological Research, Yokohama City UniversityYokohama, Japan.
Front Plant Sci. 2017 Jul 3;8:1001. doi: 10.3389/fpls.2017.01001. eCollection 2017.
High-salinity stress considerably affects plant growth and crop yield. Thus, developing techniques to enhance high-salinity stress tolerance in plants is important. In this study, we revealed that ethanol enhances high-salinity stress tolerance in and rice. To elucidate the molecular mechanism underlying the ethanol-induced tolerance, we performed microarray analyses using seedlings. Our data indicated that the expression levels of 1,323 and 1,293 genes were upregulated by ethanol in the presence and absence of NaCl, respectively. The expression of reactive oxygen species (ROS) signaling-related genes associated with high-salinity tolerance was upregulated by ethanol under salt stress condition. Some of these genes encode ROS scavengers and transcription factors (e.g., and ). A RT-qPCR analysis confirmed that the expression levels of and as well as and , which encode cytosolic ascorbate peroxidases (APX), were higher in ethanol-treated plants than in untreated control plants, when exposure to high-salinity stress. Additionally, cytosolic APX activity increased by ethanol in response to salinity stress. Moreover, histochemical analyses with 3,3'-diaminobenzidine (DAB) and nitro blue tetrazolium (NBT) revealed that ROS accumulation was inhibited by ethanol under salt stress condition in and rice, in which DAB staining data was further confirmed by Hydrogen peroxide (HO) content. These results suggest that ethanol enhances high-salinity stress tolerance by detoxifying ROS. Our findings may have implications for improving salt-stress tolerance of agriculturally important field-grown crops.
高盐胁迫严重影响植物生长和作物产量。因此，开发提高植物耐高盐胁迫能力的技术至关重要。在本研究中，我们发现乙醇可提高拟南芥和水稻的耐高盐胁迫能力。为阐明乙醇诱导耐受性的分子机制，我们对拟南芥幼苗进行了微阵列分析。我们的数据表明，在有和没有NaCl的情况下，分别有1323个和1293个基因的表达水平被乙醇上调。在盐胁迫条件下，与高盐耐受性相关的活性氧（ROS）信号相关基因的表达被乙醇上调。其中一些基因编码ROS清除剂和转录因子（如和）。逆转录定量聚合酶链反应（RT-qPCR）分析证实，在暴露于高盐胁迫时，乙醇处理的植物中编码胞质抗坏血酸过氧化物酶（APX）的和以及和的表达水平高于未处理的对照植物。此外，乙醇可使胞质APX活性在盐胁迫下增加。此外，用3,3'-二氨基联苯胺（DAB）和氮蓝四唑（NBT）进行的组织化学分析表明，在盐胁迫条件下，乙醇可抑制拟南芥和水稻中的ROS积累，其中过氧化氢（H₂O₂）含量进一步证实了DAB染色数据。这些结果表明，乙醇通过清除ROS来提高耐高盐胁迫能力。我们的发现可能对提高农业上重要的田间种植作物的耐盐胁迫能力具有启示意义。
