Joseph S A, Piekut D T
Am J Anat. 1986 Feb-Mar;175(2-3):331-42, 356-61. doi: 10.1002/aja.1001750215.
A method for sequential immunostaining of two antigens in the same section and the application to neuronal elements in the CNS is described. This method employs the peroxidase-antiperoxidase (PAP) technique as a first sequence, followed by glucose oxidase-antiglucose oxidase (GAG) complex as the second sequence, yielding rich brown and vibrant blue reaction products, respectively. Application of the PAP/GAG immunoreagents as dual markers for various neuropeptide and neurotransmitter systems is described in detail and the procedure is outlined. Comparisons with other dual staining methods are discussed and the application to other systems is presented.
描述了一种在同一组织切片中对两种抗原进行连续免疫染色的方法及其在中枢神经系统神经元成分中的应用。该方法首先采用过氧化物酶-抗过氧化物酶（PAP）技术，然后采用葡萄糖氧化酶-抗葡萄糖氧化酶（GAG）复合物作为第二步，分别产生丰富的棕色和鲜艳的蓝色反应产物。详细描述了将PAP/GAG免疫试剂用作各种神经肽和神经递质系统的双重标记物的应用，并概述了操作步骤。讨论了与其他双重染色方法的比较，并介绍了该方法在其他系统中的应用。
