Vahabi Surena, Yadegari Zahra, Mohammad-Rahimi Hossein
Periodontics Department, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Departments of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cell Tissue Bank. 2017 Sep;18(3):347-353. doi: 10.1007/s10561-017-9640-7. Epub 2017 Jul 17.
Platelet-rich plasma (PRP) contains growth factors which positively affect cell proliferation, cell differentiation, chemotaxis and intracellular matrix synthesis. All these processes are involved in wound healing and tissue regeneration; thus, PRP as a source of growth factors can be used in periodontal regenerative therapies. The purpose of the present study was to assess the effect of various concentrations of activated and non-activated PRP on proliferation of osteoblasts and fibroblasts in vitro. PRP was obtained from three healthy volunteers. 75, 50, 25, and 10% concentrations of f PRP were prepared by dilution in Dulbecco's modified Eagle's medium. In activated PRP groups, PRP concentrations were activated by adding calcium gluconate. Human gingival fibroblast (HGF) cell line and MG-63 (osteosarcoma) human osteoblast-like cell line were used in the study. The MTT proliferation assay was used to assess the effect of different types of PRP concentrates on proliferation of HGF and MG-63 cells, in 24, 48 and 72 h. After 24, 48, and 72 h, the proliferation rate of both cell lines was higher in the positive control group, except in 72 h in HGF cell lines, that 10% non-activated PRP group and 10 and 25% activated PRP groups has higher proliferation rate than the positive control group, which it was not significant. Proliferation rate in cells with 10% activated PRP was highest among samples containing PRP. The current study failed to show the significant effect of activated or non-activated PRP on proliferation of HGFs or MG-63 osteoblast-like cells. However, our results showed that activated PRP had a greater effect than non-activated PRP.
富血小板血浆（PRP）含有能对细胞增殖、细胞分化、趋化性和细胞内基质合成产生积极影响的生长因子。所有这些过程都参与伤口愈合和组织再生；因此，PRP作为生长因子的来源可用于牙周再生治疗。本研究的目的是评估不同浓度的活化和未活化PRP对体外成骨细胞和成纤维细胞增殖的影响。PRP取自三名健康志愿者。通过在杜尔贝科改良伊格尔培养基中稀释制备75%、50%、25%和10%浓度的PRP。在活化PRP组中，通过添加葡萄糖酸钙来活化PRP浓度。本研究使用了人牙龈成纤维细胞（HGF）细胞系和MG-63（骨肉瘤）人成骨样细胞系。采用MTT增殖试验评估不同类型的PRP浓缩物在24、48和72小时对HGF和MG-63细胞增殖的影响。在24、48和72小时后，除了在HGF细胞系的72小时时，阳性对照组中两种细胞系的增殖率更高外，10%未活化PRP组以及10%和25%活化PRP组的增殖率高于阳性对照组，但差异不显著。在含PRP的样本中，10%活化PRP处理的细胞增殖率最高。目前的研究未能显示活化或未活化PRP对HGF或MG-63成骨样细胞增殖有显著影响。然而，我们的结果表明活化PRP比未活化PRP的作用更大。
