Department of Endocrinology, Qingdao Municipal Hospital, 5 Donghai Street, Qingdao 266071, Shandong, China.
Department of Cardiology, Shandong Electric Power Central Hospital, 117 Jingshi Street, Jinan, China.
J Diabetes Complications. 2017 Sep;31(9):1363-1369. doi: 10.1016/j.jdiacomp.2017.06.011. Epub 2017 Jun 30.
The proposed mechanisms of impaired wound healing in diabetes involve sustained inflammation, excess oxidative stress and compromised agiogenesis. Hydrogen sulfide (HS) has been reported to have multiple biological activities. We aim to investigate the role of HS in impaired wound healing in ob/ob mice and explore the possible mechanisms involved.
Full-thickness skin dorsal wounds were created on ob/ob mice and C57BL/6 mice. Cystathionine-γ-lyase (CSE) expression and HS production were determined in granulation tissues of the wounds. Effects of NaHS on wound healing were evaluated. Inflammation and angiogenesis in granulation tissues of the wounds were examined.
CSE expression, and HS content were significantly reduced in granulation tissues of wounds in ob/ob mice compared with control mice. NaHS treatment significantly improved wound healing in ob/ob mice, which was associated with reduced neutrophil and macrophage infiltration, decreased production of tumor necrosis factor (TNF)-α, interleukin (IL)-6. NaHS treatment decreased metalloproteinase (MMP)-9, whereas increased collagen deposition and vascular-like structures in granulation tissues of wounds in ob/ob mice.
CSE down-regulation may play a role in the pathogenesis of diabetic impaired wound healing. Exogenous HS could be a potential agent to improve diabetic impaired wound healing by attenuating inflammation and increasing angiogenesis.
糖尿病导致伤口愈合受损的机制涉及持续的炎症、氧化应激过度和血管生成受损。硫化氢（HS）具有多种生物学活性。我们旨在研究 HS 在 ob/ob 小鼠伤口愈合受损中的作用，并探讨涉及的可能机制。
在 ob/ob 小鼠和 C57BL/6 小鼠的背部皮肤全层造成创伤。测定创伤肉芽组织中胱硫醚-γ-裂解酶（CSE）的表达和 HS 的产生。评估 NaHS 对伤口愈合的影响。检查创伤肉芽组织中的炎症和血管生成。
与对照组小鼠相比，ob/ob 小鼠创伤肉芽组织中的 CSE 表达和 HS 含量明显降低。NaHS 治疗显著改善了 ob/ob 小鼠的伤口愈合，这与中性粒细胞和巨噬细胞浸润减少、肿瘤坏死因子（TNF）-α、白细胞介素（IL）-6 产生减少有关。NaHS 治疗降低了基质金属蛋白酶（MMP）-9，而增加了 ob/ob 小鼠创伤肉芽组织中的胶原沉积和类似血管的结构。
CSE 的下调可能在糖尿病伤口愈合受损的发病机制中起作用。外源性 HS 可能通过减轻炎症和增加血管生成来改善糖尿病伤口愈合受损。
