Zhao Xiaoqing, Chen Yao, Lin Zhongxiao, Jin Xinyang, Su Bolun, Liu Xiaotong, Yang Mao, Chen Keyuan, Zhu Menglin, Wang Lei, Zhu Yi Zhun
State Key Laboratory of Quality Research in Chinese Medicine, Faculty of Chinese Medicine Macau University of Science and Technology Macau China.
Department of Medical Cosmetology Affiliated Hospital of Nantong University Nantong Jiangsu China.
MedComm (2020). 2024 Mar 2;5(3):e485. doi: 10.1002/mco2.485. eCollection 2024 Mar.
Hydrogen sulfide for wound healing has drawn a lot of attention recently. In this research, the S-propargyl-cysteine (SPRC), an endogenous HS donor, was loaded on carbomer hydrogel, and a copper sheet rat burn model was developed. Pathological changes in rat skin tissue were examined using hematoxylin-eosin (HE) and Masson staining. The immunohistochemistry (IHC) staining was performed to detect the expression of Collagen I (Col I) and Collagen III (Col III). The mRNA levels of interleukin (IL)-6, Col Iα2, Col IIIα1, tissue inhibitors of metalloproteinase (TIMP)-1, matrix metalloproteinase (MMP)-9, vascular endothelial growth factor (VEGF), and transforming growth factor (TGF)-β1 were examined by quantitative real-time chain polymerase reaction. The findings demonstrated that the collagen layer was thicker in the SPRC group during the proliferative phase, SPRC hydrogel promoted VEGF expression. In the late stage of wound healing, the expression of IL-6, TIMP-1, MMP-9, and TGF-β1 was inhibited, and the Col I content was closer to that of normal tissue. These results surface that SPRC hydrogel can promote wound healing and play a positive role in reducing scar formation. Our results imply that SPRC can facilitate wound healing and play a positive role in reducing scar formation.
近年来，硫化氢在伤口愈合方面备受关注。在本研究中，将内源性硫化氢供体S-炔丙基半胱氨酸（SPRC）负载于卡波姆水凝胶上，并建立了大鼠铜片烧伤模型。采用苏木精-伊红（HE）染色和Masson染色检测大鼠皮肤组织的病理变化。进行免疫组织化学（IHC）染色以检测Ⅰ型胶原（ColⅠ）和Ⅲ型胶原（ColⅢ）的表达。通过定量实时链聚合酶反应检测白细胞介素（IL）-6、ColⅠα2、ColⅢα1、金属蛋白酶组织抑制剂（TIMP）-1、基质金属蛋白酶（MMP）-9、血管内皮生长因子（VEGF）和转化生长因子（TGF）-β1的mRNA水平。研究结果表明，在增殖期，SPRC组的胶原层更厚，SPRC水凝胶促进了VEGF的表达。在伤口愈合后期，IL-6、TIMP-1、MMP-9和TGF-β1的表达受到抑制，ColⅠ含量更接近正常组织。这些结果表明，SPRC水凝胶可促进伤口愈合，并在减少瘢痕形成方面发挥积极作用。我们的结果表明，SPRC可促进伤口愈合，并在减少瘢痕形成方面发挥积极作用。
