Cannon B, Nedergaard J, Lundberg J M, Hökfelt T, Terenius L, Goldstein M
Exp Cell Res. 1986 Jun;164(2):546-50. doi: 10.1016/0014-4827(86)90052-2.
By using immunohistochemistry it is shown that both the parenchymal and vascular sympathetic innervation in the interscapular depot of brown adipose tissue in the rat contain the catecholamine-synthesizing enzyme tyrosine-hydroxylase (TH). In contrast, 'neuropeptide tyrosine' (NPY) is selectively present in the vascular sympathetic nerves of the tissue--but not in nerves around brown fat cells. This is consistent with the presence of two populations of neurons (containing either TH alone or TH plus NPY) in the stellate ganglion, which is the probable origin of the sympathetic nerves in the interscapular brown adipose tissue. Furthermore, the perivascular NPY-positive nerves in the brown adipose tissue disappeared after 6-hydroxydopamine treatment, demonstrating their noradrenergic nature. Taken together, these findings suggest that sympathetic nerves to blood vessels and brown fat cells represent two separate subpopulations of autonomic neurons.
通过免疫组织化学方法显示，大鼠肩胛间棕色脂肪组织储存部位的实质和血管交感神经支配均含有儿茶酚胺合成酶酪氨酸羟化酶（TH）。相比之下，“神经肽Y”（NPY）选择性地存在于该组织的血管交感神经中，而不存在于棕色脂肪细胞周围的神经中。这与星状神经节中存在两类神经元（一类仅含TH，另一类含TH加NPY）相一致，星状神经节可能是肩胛间棕色脂肪组织交感神经的起源。此外，棕色脂肪组织中血管周围NPY阳性神经在经6-羟基多巴胺处理后消失，证明了它们的去甲肾上腺素能性质。综上所述，这些发现表明支配血管和棕色脂肪细胞的交感神经代表了自主神经元的两个独立亚群。
