Bidmon H J, Stumpf W E, Kawamata S, Shirasu K, Gutkowska J, Sar M
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599-7090.
Histochemistry. 1990;94(5):505-8. doi: 10.1007/BF00272614.
The distribution of estrogen target neurons which contain atrial natriuretic factor (ANF) in female rat hypothalamus was investigated by thaw-mount auto-radiography combined with immunocytochemistry using tritium-labeled estradiol and antibodies against ANF. Colocalization of the two hormones was found in the arcuate nucleus, periventricular nucleus, lateral ventromedial nucleus, ventral premammillar nucleus and lateral basal hypothalamus. The percentage of ANF containing cells which concentrate estradiol varies among the different hypothalamic nuclei with the highest number of ANF-positive cells showing nuclear concentration of 3H-estradiol (80-90%) in the nucleus premammillaris ventralis, but less (5-15%) in the other nuclei. These data, together with topographical correspondence in extrahypothalamic brain regions between sites of action of estradiol and production of ANF, suggest extensive interrelationships and modulatory effects of estradiol on ANF production and secretion in the brain, similar to the atrium of the heart.
采用氚标记的雌二醇和抗心钠素抗体，通过冻融涂片放射自显影结合免疫细胞化学方法，研究了雌性大鼠下丘脑含心钠素（ANF）的雌激素靶神经元的分布。在弓状核、室周核、腹内侧外侧核、乳头体前腹侧核和下丘脑外侧基底部发现了两种激素的共定位。含ANF且浓集雌二醇的细胞百分比在不同下丘脑核团中有所不同，其中乳头体前腹侧核中ANF阳性细胞显示核浓集3H-雌二醇的数量最多（80-90%），而在其他核团中较少（5-15%）。这些数据，连同下丘脑外脑区中雌二醇作用部位与ANF产生部位之间的拓扑对应关系，提示雌二醇对脑内ANF产生和分泌具有广泛的相互关系和调节作用，类似于心脏的心房。
